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Invitrogen
KCNQ4 Monoclonal Antibody (N43/6), FITC
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FIGURE: 1 / 3
KCNQ4 Antibody (MA5-45412) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with KCNQ4 monoclonal antibody (Product # MA5-45412) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCNQ4 antibody (D) Composite.
Product Details
MA5-45412
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N43/6
Immunogen
Fusion protein amino acids 2-77 of human KCNQ4
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931866
Product Specific Information
1 µg/mL of MA5-45412 was sufficient for detection of KCNQ4 in 10 µg of COS-1 cell lysate transiently expressing KCNQ4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 77kDa.
Target Information
The protein encoded by this gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability, particularly in sensory cells of the cochlea. The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug. The encoded protein can form a homomultimeric potassium channel or possibly a heteromultimeric channel in association with the protein encoded by the KCNQ3 gene. Defects in this gene are a cause of nonsyndromic sensorineural deafness type 2, an autosomal dominant form of progressive hearing loss. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DFNA 2; KCNQ 4; KQT like 4; KQT-like 4; potassium channel KQT-like 4; Potassium channel subunit alpha KvLQT4; potassium channel, voltage gated KQT-like subfamily Q, member 4; Potassium voltage-gated channel subfamily KQT member 4; potassium voltage-gated channel, KQT-like subfamily, member 4; potassium voltage-gated channel, subfamily Q, member 4; Voltage-gated potassium channel subunit Kv7.4
Gene Aliases: DFNA2; DFNA2A; KCNQ4; KV7.4
UniProt ID: (Human) P56696, (Mouse) Q9JK97
Entrez Gene ID: (Human) 9132, (Mouse) 60613, (Rat) 298496
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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