Invitrogen

KDEL Polyclonal Antibody

KDEL Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

KDEL Antibody (PA1-013) in ICC/IF

Immunofluorescent analysis of KDEL in U251 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a KDEL polyclonal antibody (Product # PA1-013) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). KDEL staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

KDEL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KDEL Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

KDEL Antibody in Immunohistochemistry (IHC)

Product Image Gallery

KDEL Antibody — FIGURE 5. Differential VCAM-1 production between murine airway smooth muscle and epithelial cells in response to poly(I,C). Airway smooth muscle and epithelial cells were treated without ( NT ; lanes 1 and 4 ) or with poly(I,C) ( PIC ; lanes 2 and 5 ) or tunicamycin ( TUN ; lanes 3 and 6 ). These blots were probed with antibodies against KDEL ( A, green ) and VCAM-1 ( B, green ). Molecular weight ladders are shown in red / yellow . beta-Actin ( red ) is shown for each lane as a loading control. The KDEL and VCAM bands ( A and B , respectively) were quantified and presented in C and D , respectively. Error bars , S.D. ( n = 3). {TM}

Product Details

PA1-013

Applications

Tested Dilution

Publications

Western Blot (WB)

8-16 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

Immunocytochemistry (ICC/IF)

Assay-dependent

View 11 publications 11 publications

Flow Cytometry (Flow)

1-20 µg/mL

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Hamster, Human, Mouse, Rat, Sea urchin

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325593

Product Specific Information

PA1-013 detects KDEL from human, rat, mouse and hamster.

PA1-013 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 57 kDa protein representing protein disulfide isomerase (PDI), a 78 kDa protein representing glucose regulated protein 70 kDa (GRP78) and an ~94 kDa protein representing GRP94 in mouse myeloma cells. Immunofluorescence staining of GRP78 in mouse myeloma cells with PA1-013 results in diffuse cytoplasmic staining consistent with specific ER localization. PA1-013 immunoprecipitates PDI, GRP78, an ~72 kDa protein which may be endoplasmic reticulum protein 72 kDa (ERp72), and an ~94 kDa protein representing GRP94 from numerous rodent tissues.

The PA1-013 immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.

Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.

Target Information

The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dependence of ABCB1 transporter expression and function on distinct sphingolipids generated by ceramide synthases-2 and -6 in chemoresistant renal cancer.

The Journal of biological chemistry

Lee WK,Maaß M,Quach A,Poscic N,Prangley H,Pallott EC,Kim JL,Pierce JS,Ogretmen B,Futerman AH,Thévenod F

PA1-013 was used in Immunocytochemistry to conclude that a critical balance in ceramide/SM species is prerequisite to ABCB1 expression and functionalization, which could be targeted to reverse multidrug resistance in renal cancers.

Tue Feb 01 00:00:00 UTC 2022

Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells.

Nature communications

Della-Flora Nunes G,Wilson ER,Marziali LN,Hurley E,Silvestri N,He B,O'Malley BW,Beirowski B,Poitelon Y,Wrabetz L,Feltri ML

PA1-013 was used in Immunohistochemistry to elucidate the functional contribution of prohibitin 1 in schwann cells.

Wed Jun 02 00:00:00 UTC 2021

Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells.

Nature communications

Della-Flora Nunes G,Wilson ER,Marziali LN,Hurley E,Silvestri N,He B,O'Malley BW,Beirowski B,Poitelon Y,Wrabetz L,Feltri ML

PA1-013 was used in Immunohistochemistry to elucidate the functional contribution of prohibitin 1 in schwann cells.

Wed Jun 02 00:00:00 UTC 2021

Induction of Axonal Outgrowth in Mouse Hippocampal Neurons via Bacterial Magnetosomes.

International journal of molecular sciences

De Vincentiis S,Falconieri A,Mickoleit F,Cappello V,Schüler D,Raffa V

PA1-013 was used in Immunocytochemistry-immunoflourescence to data show that bacterial magnetosomes are more efficient than synthetic iron oxide nanoparticles in stimulating axonal outgrowth via SG.

Fri Apr 16 00:00:00 UTC 2021

Induction of Axonal Outgrowth in Mouse Hippocampal Neurons via Bacterial Magnetosomes.

International journal of molecular sciences

De Vincentiis S,Falconieri A,Mickoleit F,Cappello V,Schüler D,Raffa V

PA1-013 was used in Immunocytochemistry-immunoflourescence to data show that bacterial magnetosomes are more efficient than synthetic iron oxide nanoparticles in stimulating axonal outgrowth via SG.

Fri Apr 16 00:00:00 UTC 2021

Posttranslational modifications of serine protease TMPRSS13 regulate zymogen activation, proteolytic activity, and cell surface localization.

The Journal of biological chemistry

Martin CE,Murray AS,Sala-Hamrick KE,Mackinder JR,Harrison EC,Lundgren JG,Varela FA,List K

Published figure using KDEL polyclonal antibody (Product # PA1-013) in Immunocytochemistry

Fri Oct 01 00:00:00 UTC 2021

Dopamine Transporter Localization in Medial Forebrain Bundle Axons Indicates Its Long-Range Transport Primarily by Membrane Diffusion with a Limited Contribution of Vesicular Traffic on Retromer-Positive Compartments.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Bagalkot TR,Block ER,Bucchin K,Balcita-Pedicino JJ,Calderon M,Sesack SR,Sorkin A

PA1-013 was used in Immunohistochemistry to suggest that DAT traffics between midbrain and striatum mainly by lateral diffusion in the plasma membrane with only a limited contribution of vesicular transport in recycling endosomes.

Wed Jan 13 00:00:00 UTC 2021

Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight.

Methods in molecular biology (Clifton, N.J.)

Hernández-Pérez S,Runsala M,Šuštar V,Mattila PK

PA1-013 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot to provide a protocol for 3D analysis of the intracellular vesicle traffic, in either fixed or living cells, using B lymphocytes (B cells) as a model system.

Wed Aug 11 00:00:00 UTC 2021

Ankyrin Is An Intracellular Tether for TMC Mechanotransduction Channels.

Neuron

Tang YQ,Lee SA,Rahman M,Vanapalli SA,Lu H,Schafer WR

PA1-013 was used in Immunocytochemistry to identify UNC-44/ankyrin as an essential component of the TMC-1 mechanotransduction channel complex in the sensory cilia of Caenorhabditis elegans mechanoreceptor neurons.

Wed Jul 08 00:00:00 UTC 2020

The Perinuclear ER Scales Nuclear Size Independently of Cell Size in Early Embryos.

Developmental cell

Mukherjee RN,Sallé J,Dmitrieff S,Nelson KM,Oakey J,Minc N,Levy DL

PA1-013 was used in Immunocytochemistry to monitor nuclear dynamics in early sea urchin embryos and find that nuclei undergo substantial growth in each interphase, reaching a maximal size prior to mitosis that declined steadily over the course of development.

Mon Aug 10 00:00:00 UTC 2020

Extremely Low Forces Induce Extreme Axon Growth.

The Journal of neuroscience : the official journal of the Society for Neuroscience

De Vincentiis S,Falconieri A,Mainardi M,Cappello V,Scribano V,Bizzarri R,Storti B,Dente L,Costa M,Raffa V

PA1-013 was used in Immunocytochemistry to generate a dragging force using a protocol based on magnetic nanoparticles (NPs).

Wed Jun 24 00:00:00 UTC 2020

Extremely Low Forces Induce Extreme Axon Growth.

The Journal of neuroscience : the official journal of the Society for Neuroscience

De Vincentiis S,Falconieri A,Mainardi M,Cappello V,Scribano V,Bizzarri R,Storti B,Dente L,Costa M,Raffa V

PA1-013 was used in Immunocytochemistry to generate a dragging force using a protocol based on magnetic nanoparticles (NPs).

Wed Jun 24 00:00:00 UTC 2020

TUBB1 mutations cause thyroid dysgenesis associated with abnormal platelet physiology.

EMBO molecular medicine

Stoupa A,Adam F,Kariyawasam D,Strassel C,Gawade S,Szinnai G,Kauskot A,Lasne D,Janke C,Natarajan K,Schmitt A,Bole-Feysot C,Nitschke P,Léger J,Jabot-Hanin F,Tores F,Michel A,Munnich A,Besmond C,Scharfmann R,Lanza F,Borgel D,Polak M,Carré A

PA1-013 was used in Immunohistochemistry to identify three novel TUBB1 gene mutations causing thyroid dysgenesis.

Sat Dec 01 00:00:00 UTC 2018

TUBB1 mutations cause thyroid dysgenesis associated with abnormal platelet physiology.

EMBO molecular medicine

PA1-013 was used in Immunohistochemistry to identify three novel TUBB1 gene mutations causing thyroid dysgenesis.

Sat Dec 01 00:00:00 UTC 2018

CIB2 interacts with TMC1 and TMC2 and is essential for mechanotransduction in auditory hair cells.

Nature communications

Giese APJ,Tang YQ,Sinha GP,Bowl MR,Goldring AC,Parker A,Freeman MJ,Brown SDM,Riazuddin S,Fettiplace R,Schafer WR,Frolenkov GI,Ahmed ZM

PA1-013 was used in Immunocytochemistry-immunoflourescence to study the exact functions of calcium and integrin-binding protein 2.

Thu Jun 29 00:00:00 UTC 2017

Axons provide the secretory machinery for trafficking of voltage-gated sodium channels in peripheral nerve.

Proceedings of the National Academy of Sciences of the United States of America

González C,Cánovas J,Fresno J,Couve E,Court FA,Couve A

PA1-013 was used in immunocytochemistry to characterize the secretory machinery in axons and study its contribution to plasma membrane delivery of sodium channels

Tue Feb 16 00:00:00 UTC 2016

Hyaluronan Rafts on Airway Epithelial Cells.

The Journal of biological chemistry

Abbadi A,Lauer M,Swaidani S,Wang A,Hascall V

Published figure using KDEL polyclonal antibody (Product # PA1-013) in Immunocytochemistry

Fri Jan 15 00:00:00 UTC 2016

Hyaluronan Rafts on Airway Epithelial Cells.

The Journal of biological chemistry

Abbadi A,Lauer M,Swaidani S,Wang A,Hascall V

Published figure using KDEL polyclonal antibody (Product # PA1-013) in Immunocytochemistry

Fri Jan 15 00:00:00 UTC 2016

Primary murine airway smooth muscle cells exposed to poly(I,C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix.

The Journal of biological chemistry

Lauer ME,Mukhopadhyay D,Fulop C,de la Motte CA,Majors AK,Hascall VC

PA1-013 was used in western blot to study the response of primary murine airway smooth muscle cells to poly (I,C) or tunicamycin

Fri Feb 20 00:00:00 UTC 2009

Primary murine airway smooth muscle cells exposed to poly(I,C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix.

The Journal of biological chemistry

Lauer ME,Mukhopadhyay D,Fulop C,de la Motte CA,Majors AK,Hascall VC

PA1-013 was used in western blot to study the response of primary murine airway smooth muscle cells to poly (I,C) or tunicamycin

Fri Feb 20 00:00:00 UTC 2009

Differentiated murine airway epithelial cells synthesize a leukocyte-adhesive hyaluronan matrix in response to endoplasmic reticulum stress.

The Journal of biological chemistry

Lauer ME,Erzurum SC,Mukhopadhyay D,Vasanji A,Drazba J,Wang A,Fulop C,Hascall VC

PA1-013 was used in western blot to examine hyaluronan synthesis by the airway epithelial cells in response to endoplasmic reticulum stress.

Fri Sep 19 00:00:00 UTC 2008

Differentiated murine airway epithelial cells synthesize a leukocyte-adhesive hyaluronan matrix in response to endoplasmic reticulum stress.

The Journal of biological chemistry

Lauer ME,Erzurum SC,Mukhopadhyay D,Vasanji A,Drazba J,Wang A,Fulop C,Hascall VC

PA1-013 was used in western blot to examine hyaluronan synthesis by the airway epithelial cells in response to endoplasmic reticulum stress.

Fri Sep 19 00:00:00 UTC 2008

Differentiated murine airway epithelial cells synthesize a leukocyte-adhesive hyaluronan matrix in response to endoplasmic reticulum stress.

The Journal of biological chemistry

Lauer ME,Erzurum SC,Mukhopadhyay D,Vasanji A,Drazba J,Wang A,Fulop C,Hascall VC

PA1-013 was used in western blot to examine hyaluronan synthesis by the airway epithelial cells in response to endoplasmic reticulum stress.

Fri Sep 19 00:00:00 UTC 2008

Differentiated murine airway epithelial cells synthesize a leukocyte-adhesive hyaluronan matrix in response to endoplasmic reticulum stress.

The Journal of biological chemistry

Lauer ME,Erzurum SC,Mukhopadhyay D,Vasanji A,Drazba J,Wang A,Fulop C,Hascall VC

PA1-013 was used in western blot to examine hyaluronan synthesis by the airway epithelial cells in response to endoplasmic reticulum stress.

Fri Sep 19 00:00:00 UTC 2008

Further characterization of mammalian ceramide kinase: substrate delivery and (stereo)specificity, tissue distribution, and subcellular localization studies.

Journal of lipid research

Van Overloop H,Gijsbers S,Van Veldhoven PP

PA1-013 was used in immunocytochemistry to investigate related factors affecting recombinant human ceramide kinase's activity

Wed Feb 01 00:00:00 UTC 2006

Changes of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding alpha1-antitrypsin transgenic mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Papp E,Száraz P,Korcsmáros T,Csermely P

PA1-013 was used in western blot to investigate the changes of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding alpha1-antitrypsin transgenic mice

Mon May 01 00:00:00 UTC 2006

Functional proteomic screens reveal an essential extracellular role for hsp90 alpha in cancer cell invasiveness.

Nature cell biology

Eustace BK,Sakurai T,Stewart JK,Yimlamai D,Unger C,Zehetmeier C,Lain B,Torella C,Henning SW,Beste G,Scroggins BT,Neckers L,Ilag LL,Jay DG

PA1-013 was used in immunoprecipitation to study the role of extracellular Hsp90-alpha in the activation of MMP2 and invasiveness of fibrosarcoma cells

Tue Jun 01 00:00:00 UTC 2004

Protein-disulfide isomerase-mediated reduction of the A subunit of cholera toxin in a human intestinal cell line.

The Journal of biological chemistry

Orlandi PA

PA1-013 was used in western blot to study the mechanisms of cholera toxin A subunit reduction in a human intestinal cell line

Fri Feb 14 00:00:00 UTC 1997

Bioinformatics

Protein Aliases: 78 kDa glucose-regulated protein; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Binding-immunoglobulin protein; BiP; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; epididymis secretory sperm binding protein Li 89n; ER lumen protein retaining receptor 1; ER lumen protein-retaining receptor 1; ERp99; glucose regulated protein, 78 kDa; glucose-regulated protein; glucose-regulated protein, 78kDa; gp96 homolog; GRP 78; GRP-78; GRP-94; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 5; Heat shock protein 70 family protein 5; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90, beta (Grp94), member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family A member 5; Heat shock protein family C member 4; HSP70 family protein 5; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Immunoglobulin heavy chain-binding protein; KDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Lys-Asp-Glu-Leu; Polymorphic tumor rejection antigen 1; Putative MAPK-activating protein PM23; RP23-446N16.1; Steroidogenesis-activator polypeptide; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; XAP-1 antigen

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Gene Aliases: 8030486F04Rik; AL022860; AU019543; AW215843; baffled; BIP; D2Wsu141e; D2Wsu17e; ECGP; endoplasmin; ERD2; ERD2.1; ERp99; GP96; GRP78; GRP94; HDEL; HEL-S-125m; HEL-S-89n; HEL35; Hsce70; HSP90B1; HSPA5; HSPC4; I79_019946; I79_020931; KDELR1; mBiP; MIF2; PM23; SEZ-7; Sez7; TA-3; Targ2; Tra-1; TRA1

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UniProt ID: (Human) P14625, (Human) P11021, (Mouse) P08113, (Rat) Q66HD0, (Rat) P06761, (Mouse) P20029, (Chinese hamster) G3I8R9, (Human) P24390, (Mouse) Q99JH8, (Rat) Q569A6

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Entrez Gene ID: (Human) 7184, (Human) 3309, (Chinese hamster) 100773331, (Mouse) 22027, (Rat) 362862, (Rat) 25617, (Mouse) 14828, (Chinese hamster) 100689305, (Human) 10945, (Mouse) 68137, (Rat) 361577, (Chinese hamster) 100750912

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Function(s)

RNA binding calcium ion binding protein binding ATP binding protein phosphatase binding virion binding low-density lipoprotein particle receptor binding unfolded protein binding glycoprotein binding ATPase activity enzyme binding protein domain specific binding ubiquitin protein ligase binding ribosome binding chaperone binding misfolded protein binding cadherin binding involved in cell-cell adhesion nucleotide binding KDEL sequence binding molecular_function ER retention sequence binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KDEL Polyclonal Antibody

KDEL Antibody (PA1-013) in ICC/IF

Product Details

PA1-013

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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