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KDM5A Antibody (PA5-103448) in IHC (P)
Immunohistochemistry analysis of KDM5A in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with KDM5A polyclonal antibody (Product # PA5-103448) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-103448
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KDM5A(Accession P29375), corresponding to amino acid residues E1384-V1434.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852789
Product Specific Information
Antibody detects endogenous levels of total KDM5A.
Target Information
UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctionsThis gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [histone H3]-trimethyl-L-lysine(4) demethylase 5A; Histone demethylase JARID1A; jumonji, AT rich interactive domain 1A (Rbp2 like); Jumonji, AT rich interactive domain 1A (RBP2-like); Jumonji/ARID domain-containing protein 1A; lysine (K)-specific demethylase 5A; Lysine-specific demethylase 5A; RBBP-2; retinoblastoma binding protein 2; Retinoblastoma-binding protein 2
Gene Aliases: AA409370; C76986; JARID1A; KDM5A; RBBP-2; RBBP2; RBP2
UniProt ID: (Human) P29375, (Mouse) Q3UXZ9
Entrez Gene ID: (Human) 5927, (Mouse) 214899
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
zinc ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
chromatin DNA binding
histone demethylase activity
sulfonate dioxygenase activity
chromatin binding
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
metal ion binding
dioxygenase activity
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
chromatin modification
circadian regulation of gene expression
positive regulation of transcription, DNA-templated
rhythmic process
oxidation-reduction process
negative regulation of histone deacetylase activity
regulation of nucleic acid-templated transcription
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
spermatogenesis
male gonad development
histone H3-K4 demethylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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