Proteintech

KGA Polyclonal Antibody

KGA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

KGA Antibody (20170-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HEK-293 cells using 20170-1-AP (KGA-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

KGA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KGA Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20170-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:400

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KGA-Specific Fusion Protein Ag14002 (616-669 aa encoded by BC038507)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.71 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific to KGA.

Immunogen sequence: KDRWNNTPM DEALHFGHHD VFKILQEYQV QYTPQGDSDN GKENQTVHKN LDGLL (616-669 aa encoded by BC038507)

Target Information

Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of glutaminase 1-mediated glutaminolysis improves pathological cardiac remodeling.

American journal of physiology. Heart and circulatory physiology

Yoshikawa S,Nagao M,Toh R,Shinohara M,Iino T,Irino Y,Nishimori M,Tanaka H,Satomi-Kobayashi S,Ishida T,Hirata KI

Sun May 01 00:00:00 UTC 2022

CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.

Cell stem cell

Russo GL,Sonsalla G,Natarajan P,Breunig CT,Bulli G,Merl-Pham J,Schmitt S,Giehrl-Schwab J,Giesert F,Jastroch M,Zischka H,Wurst W,Stricker SH,Hauck SM,Masserdotti G,Götz M

Thu Mar 04 00:00:00 UTC 2021

Depletion of Intracellular Glutamine Pools Triggers Toxoplasma gondii Stage Conversion in Human Glutamatergic Neurons.

Frontiers in cellular and infection microbiology

Bando H,Fukuda Y,Watanabe N,Olawale JT,Kato K

Tue Apr 05 00:00:00 UTC 2022

Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.

International journal of molecular sciences

Beręsewicz-Haller M,Krupska O,Bochomulski P,Dudzik D,Chęcińska A,Hilgier W,Barbas C,Zablocki K,Zablocka B

Sat Aug 07 00:00:00 UTC 2021

Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.

International journal of molecular sciences

Beręsewicz-Haller M,Krupska O,Bochomulski P,Dudzik D,Chęcińska A,Hilgier W,Barbas C,Zablocki K,Zablocka B

Sat Aug 07 00:00:00 UTC 2021

A facile and sensitive method of quantifying glutaminase binding to its inhibitor CB-839 in tissues.

Journal of genetics and genomics = Yi chuan xue bao

Chen Y,Zhao Y,Bajor DL,Wang Z,Selfridge JE

Mon Jul 20 00:00:00 UTC 2020

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

EBioMedicine

Li B,Cao Y,Meng G,Qian L,Xu T,Yan C,Luo O,Wang S,Wei J,Ding Y,Yu D

Tue Jan 01 00:00:00 UTC 2019

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

EBioMedicine

Li B,Cao Y,Meng G,Qian L,Xu T,Yan C,Luo O,Wang S,Wei J,Ding Y,Yu D

Tue Jan 01 00:00:00 UTC 2019

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

EBioMedicine

Li B,Cao Y,Meng G,Qian L,Xu T,Yan C,Luo O,Wang S,Wei J,Ding Y,Yu D

Tue Jan 01 00:00:00 UTC 2019

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

EBioMedicine

Li B,Cao Y,Meng G,Qian L,Xu T,Yan C,Luo O,Wang S,Wei J,Ding Y,Yu D

Tue Jan 01 00:00:00 UTC 2019

Evidence for Altered Glutamine Metabolism in Human Immunodeficiency Virus Type 1 Infected Primary Human CD4⁺ T Cells.

AIDS research and human retroviruses

Hegedus A,Kavanagh Williamson M,Khan MB,Dias Zeidler J,Da Poian AT,El-Bacha T,Struys EA,Huthoff H

Fri Dec 01 00:00:00 UTC 2017

Evidence for Altered Glutamine Metabolism in Human Immunodeficiency Virus Type 1 Infected Primary Human CD4⁺ T Cells.

AIDS research and human retroviruses

Hegedus A,Kavanagh Williamson M,Khan MB,Dias Zeidler J,Da Poian AT,El-Bacha T,Struys EA,Huthoff H

Fri Dec 01 00:00:00 UTC 2017

Metabolic Differences in Glutamine Utilization Lead to Metabolic Vulnerabilities in Prostate Cancer.

Scientific reports

Zacharias NM,McCullough C,Shanmugavelandy S,Lee J,Lee Y,Dutta P,McHenry J,Nguyen L,Norton W,Jones LW,Bhattacharya PK

Thu Nov 23 00:00:00 UTC 2017

Metabolic Differences in Glutamine Utilization Lead to Metabolic Vulnerabilities in Prostate Cancer.

Scientific reports

Zacharias NM,McCullough C,Shanmugavelandy S,Lee J,Lee Y,Dutta P,McHenry J,Nguyen L,Norton W,Jones LW,Bhattacharya PK

Thu Nov 23 00:00:00 UTC 2017

Metabolic Differences in Glutamine Utilization Lead to Metabolic Vulnerabilities in Prostate Cancer.

Scientific reports

Zacharias NM,McCullough C,Shanmugavelandy S,Lee J,Lee Y,Dutta P,McHenry J,Nguyen L,Norton W,Jones LW,Bhattacharya PK

Thu Nov 23 00:00:00 UTC 2017

The Progestin Receptor Interactome in the Female Mouse Hypothalamus: Interactions with Synaptic Proteins Are Isoform Specific and Ligand Dependent.

eNeuro

Acharya KD,Nettles SA,Sellers KJ,Im DD,Harling M,Pattanayak C,Vardar-Ulu D,Lichti CF,Huang S,Edwards DP,Srivastava DP,Denner L,Tetel MJ

Wed May 30 00:00:00 UTC 2018

The Progestin Receptor Interactome in the Female Mouse Hypothalamus: Interactions with Synaptic Proteins Are Isoform Specific and Ligand Dependent.

eNeuro

Acharya KD,Nettles SA,Sellers KJ,Im DD,Harling M,Pattanayak C,Vardar-Ulu D,Lichti CF,Huang S,Edwards DP,Srivastava DP,Denner L,Tetel MJ

Wed May 30 00:00:00 UTC 2018

CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function.

RNA (New York, N.Y.)

Masamha CP,Xia Z,Peart N,Collum S,Li W,Wagner EJ,Shyu AB

Wed Jun 01 00:00:00 UTC 2016

T Helper Cell Activation and Expansion Is Sensitive to Glutaminase Inhibition under Both Hypoxic and Normoxic Conditions.

PloS one

Sener Z,Cederkvist FH,Volchenkov R,Holen HL,Skålhegg BS

Wed Aug 02 00:00:00 UTC 2017

T Helper Cell Activation and Expansion Is Sensitive to Glutaminase Inhibition under Both Hypoxic and Normoxic Conditions.

PloS one

Sener Z,Cederkvist FH,Volchenkov R,Holen HL,Skålhegg BS

Wed Aug 02 00:00:00 UTC 2017

T Helper Cell Activation and Expansion Is Sensitive to Glutaminase Inhibition under Both Hypoxic and Normoxic Conditions.

PloS one

Sener Z,Cederkvist FH,Volchenkov R,Holen HL,Skålhegg BS

Wed Aug 02 00:00:00 UTC 2017

T Helper Cell Activation and Expansion Is Sensitive to Glutaminase Inhibition under Both Hypoxic and Normoxic Conditions.

PloS one

Sener Z,Cederkvist FH,Volchenkov R,Holen HL,Skålhegg BS

Wed Aug 02 00:00:00 UTC 2017

Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia.

Nature medicine

Herranz D,Ambesi-Impiombato A,Sudderth J,Sánchez-Martín M,Belver L,Tosello V,Xu L,Wendorff AA,Castillo M,Haydu JE,Márquez J,Matés JM,Kung AL,Rayport S,Cordon-Cardo C,DeBerardinis RJ,Ferrando AA

Thu Oct 01 00:00:00 UTC 2015

Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia.

Nature medicine

Herranz D,Ambesi-Impiombato A,Sudderth J,Sánchez-Martín M,Belver L,Tosello V,Xu L,Wendorff AA,Castillo M,Haydu JE,Márquez J,Matés JM,Kung AL,Rayport S,Cordon-Cardo C,DeBerardinis RJ,Ferrando AA

Thu Oct 01 00:00:00 UTC 2015

Targeting glutaminolysis has antileukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.

Blood

Jacque N,Ronchetti AM,Larrue C,Meunier G,Birsen R,Willems L,Saland E,Decroocq J,Maciel TT,Lambert M,Poulain L,Hospital MA,Sujobert P,Joseph L,Chapuis N,Lacombe C,Moura IC,Demo S,Sarry JE,Recher C,Mayeux P,Tamburini J,Bouscary D

Thu Sep 10 00:00:00 UTC 2015

Targeting glutaminolysis has antileukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.

Blood

Thu Sep 10 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: GLS; glutaminase C; Glutaminase kidney isoform, mitochondrial; glutaminase, phosphate-activated; K-glutaminase; L-glutamine amidohydrolase

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Gene Aliases: 6330442B14; AAD20; AI314027; B230365M23Rik; GAC; GAM; GLS; GLS1; Glut; KGA; KIAA0838; RATGLUT

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UniProt ID: (Human) O94925, (Mouse) D3Z7P3, (Rat) P13264

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Entrez Gene ID: (Human) 2744, (Mouse) 14660, (Rat) 24398

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Function(s)

glutaminase activity protein binding hydrolase activity

Process(es)

suckling behavior regulation of respiratory gaseous exchange by neurological system process glutamate biosynthetic process glutamine catabolic process chemical synaptic transmission cellular amino acid biosynthetic process glutamate secretion protein homotetramerization glutamine metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KGA Polyclonal Antibody

KGA Antibody (20170-1-AP) in ICC/IF

Product Details

20170-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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