Proteintech

KGA/GAM/GAC Polyclonal Antibody

3 References

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (14)

KGA/GAM/GAC Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

KGA/GAM/GAC Antibody (23549-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using (Product # 23549-1-AP) (KGA/GAM/GAC antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

KGA/GAM/GAC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KGA/GAM/GAC Antibody in Western Blot (WB)

KGA/GAM/GAC Antibody in Immunoprecipitation (IP)

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Product Details

23549-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KGA/GAM/GAC Fusion Protein Ag20354 (1-140 aa encoded by BC038507)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.49 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

GLS has 3 isoforms produced by alternative splicing and this antibody is specific to the 3 isoforms (KGA,GAM,GAC) of GLS.

Immunogen sequence: MMRLRGSGM LRDLLLRSPA GVSATLRRAQ PLVTLCRRPR GGGRPAAGPA AAARLHPWWG GGGWPAEPLA RGLSSSPSEI LQELGKGSTH PQPGVSPPAA PAAPGPKDGP GETDAFGNSE GKELVASGEN KIKQGLLPSL E (1-140 aa encoded by BC038507)

Target Information

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Molecular cell

Jiang B,Zhang J,Zhao G,Liu M,Hu J,Lin F,Wang J,Zhao W,Ma H,Zhang C,Wu C,Yao L,Liu Q,Chen X,Cao Y,Zheng Y,Zhang C,Han A,Lin D,Li Q

Thu May 19 00:00:00 UTC 2022

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Molecular cell

Jiang B,Zhang J,Zhao G,Liu M,Hu J,Lin F,Wang J,Zhao W,Ma H,Zhang C,Wu C,Yao L,Liu Q,Chen X,Cao Y,Zheng Y,Zhang C,Han A,Lin D,Li Q

Thu May 19 00:00:00 UTC 2022

Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels.

Redox biology

Smith MR,Vayalil PK,Zhou F,Benavides GA,Beggs RR,Golzarian H,Nijampatnam B,Oliver PG,Smith RA,Murphy MP,Velu SE,Landar A

Mon Aug 01 00:00:00 UTC 2016

Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels.

Redox biology

Smith MR,Vayalil PK,Zhou F,Benavides GA,Beggs RR,Golzarian H,Nijampatnam B,Oliver PG,Smith RA,Murphy MP,Velu SE,Landar A

Mon Aug 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: GLS; glutaminase C; Glutaminase kidney isoform, mitochondrial; glutaminase, phosphate-activated; K-glutaminase; L-glutamine amidohydrolase

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Gene Aliases: 6330442B14; AAD20; AI314027; B230365M23Rik; GAC; GAM; GLS; GLS1; Glut; KGA; KIAA0838; RATGLUT

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UniProt ID: (Human) O94925, (Mouse) D3Z7P3, (Rat) P13264

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Entrez Gene ID: (Human) 2744, (Mouse) 14660, (Rat) 24398

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Function(s)

glutaminase activity protein binding hydrolase activity

Process(es)

suckling behavior regulation of respiratory gaseous exchange by neurological system process glutamate biosynthetic process glutamine catabolic process chemical synaptic transmission cellular amino acid biosynthetic process glutamate secretion protein homotetramerization glutamine metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KGA/GAM/GAC Polyclonal Antibody

KGA/GAM/GAC Antibody (23549-1-AP) in ICC/IF

Product Details

23549-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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