Proteintech

KIF3A Polyclonal Antibody

KIF3A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

KIF3A Antibody (13930-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human gliomas using 13930-1-AP (KIF3A antibody) at dilution of 1:100 (under 40x lens).

KIF3A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13930-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KIF3A Fusion Protein Ag4954 (1-355 aa encoded by BC045542)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody specifically recognizes the endogenous KIF3A protein in mouse embryonic fibroblasts (MEFs) but not in KIF3A-/- lysate.

Immunogen sequence: MPINKSEKP ESCDNVKVVV RCRPLNEREK SMCYKQAVSV DEMRGTITVH KTDSSNEPPK TFTFDTVFGP ESKQLDVYNL TARPIIDSVL EGYNGTIFAY GQTGTGKTFT MEGVRAIPEL RGIIPNSFAH IFGHIAKAEG DTRFLVRVSY LGIYNEEVRD LLGKDQTQRL EVIERPDVGV YIKDLSAYVV NNADDMDRIM TLGHKNRSVG ATNMNEHSSR SHAIFTITIE CSEKGIDGNM HVRMGKLHLV DLAGSERQAK TGATGQRLKE ATKINLSLST LGNVISALVD GKSTHVPYRN SKLTRLLQDS LGGNSKTMMC ANIGPADYNY DETISTLRYA NRAKNIKNKA RINEDP (1-355 aa encoded by B C045542)

Target Information

The kinesins constitute a large family of microtubule-dependent motor proteins which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events including endocytosis and transcytosis. Members of the heterotrimeric kinesin II family of microtubule associated motors generally contain two different motor subunits from the KIF3 family, which includes KIF3A, B and C. KIF3 isoforms mediate anterograde transport of membrane bound organelles in neurons and melanosomes, transport between the endoplasmic reticulum and the Golgi, and transport of protein complexes within cilia and flagella required for their morphogenesis. KIF3A may influence neurogenesis at the level of embryonic cellular events, where the asymmetry of the genetic control circuit controlling left-right (L-R) axis determination is defined. Loss of KIF3A function in mice photoreceptors causes apoptotic cell death, suggesting that kinesin II mediated transport is required for proper cell fate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A stress-induced cilium-to-PML-NB route drives senescence initiation.

Nature communications

Ma X,Zhang Y,Zhang Y,Zhang X,Huang Y,He K,Chen C,Hao J,Zhao D,LeBrasseur NK,Kirkland JL,Chini EN,Wei Q,Ling K,Hu J

Mon Apr 03 00:00:00 UTC 2023

NEK7 promotes gastric cancer progression as a cell proliferation regulator.

Cancer cell international

Li YK,Zhu XR,Zhan Y,Yuan WZ,Jin WL

Sat Aug 21 00:00:00 UTC 2021

The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium.

Cell reports

Boukhalfa A,Roccio F,Dupont N,Codogno P,Morel E

Tue Apr 27 00:00:00 UTC 2021

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Science advances

Wilson MM,Callens C,Le Gallo M,Mironov S,Ding Q,Salamagnon A,Chavarria TE,Viel R,Peasah AD,Bhutkar A,Martin S,Godey F,Tas P,Kang HS,Juin PP,Jetten AM,Visvader JE,Weinberg RA,Attanasio M,Prigent C,Lees JA,Guen VJ

Fri Oct 29 00:00:00 UTC 2021

DAB2IP modulates primary cilia formation associated with renal tumorigenesis.

Neoplasia (New York, N.Y.)

Lin CJ,Dang A,Hernandez E,Hsieh JT

Fri Jan 01 00:00:00 UTC 2021

DAB2IP modulates primary cilia formation associated with renal tumorigenesis.

Neoplasia (New York, N.Y.)

Lin CJ,Dang A,Hernandez E,Hsieh JT

Fri Jan 01 00:00:00 UTC 2021

DAB2IP modulates primary cilia formation associated with renal tumorigenesis.

Neoplasia (New York, N.Y.)

Lin CJ,Dang A,Hernandez E,Hsieh JT

Fri Jan 01 00:00:00 UTC 2021

DAB2IP modulates primary cilia formation associated with renal tumorigenesis.

Neoplasia (New York, N.Y.)

Lin CJ,Dang A,Hernandez E,Hsieh JT

Fri Jan 01 00:00:00 UTC 2021

Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.

Cellular and molecular life sciences : CMLS

Hossain D,Barbelanne M,Tsang WY

Wed Jan 01 00:00:00 UTC 2020

Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.

Cellular and molecular life sciences : CMLS

Hossain D,Barbelanne M,Tsang WY

Wed Jan 01 00:00:00 UTC 2020

Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells.

Molecular and cellular biochemistry

Xu Q,Liu X,Liu W,Hayashi T,Yamato M,Fujisaki H,Hattori S,Tashiro SI,Onodera S,Ikejima T

Tue Jan 01 00:00:00 UTC 2019

Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells.

Molecular and cellular biochemistry

Xu Q,Liu X,Liu W,Hayashi T,Yamato M,Fujisaki H,Hattori S,Tashiro SI,Onodera S,Ikejima T

Tue Jan 01 00:00:00 UTC 2019

Roles for IFT172 and Primary Cilia in Cell Migration, Cell Division, and Neocortex Development.

Frontiers in cell and developmental biology

Pruski M,Hu L,Yang C,Wang Y,Zhang JB,Zhang L,Huang Y,Rajnicek AM,St Clair D,McCaig CD,Lang B,Ding YQ

Thu Oct 01 00:00:00 UTC 2020

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Nature communications

Tu HQ,Qin XH,Liu ZB,Song ZQ,Hu HB,Zhang YC,Chang Y,Wu M,Huang Y,Bai YF,Wang G,Han QY,Li AL,Zhou T,Liu F,Zhang XM,Li HY

Tue Dec 11 00:00:00 UTC 2018

EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.

Proceedings of the National Academy of Sciences of the United States of America

Guen VJ,Chavarria TE,Kröger C,Ye X,Weinberg RA,Lees JA

Tue Dec 05 00:00:00 UTC 2017

EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.

Proceedings of the National Academy of Sciences of the United States of America

Guen VJ,Chavarria TE,Kröger C,Ye X,Weinberg RA,Lees JA

Tue Dec 05 00:00:00 UTC 2017

Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.

The EMBO journal

Kodani A,Salomé Sirerol-Piquer M,Seol A,Garcia-Verdugo JM,Reiter JF

Wed Feb 20 00:00:00 UTC 2013

Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.

The EMBO journal

Kodani A,Salomé Sirerol-Piquer M,Seol A,Garcia-Verdugo JM,Reiter JF

Wed Feb 20 00:00:00 UTC 2013

Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.

The EMBO journal

Kodani A,Salomé Sirerol-Piquer M,Seol A,Garcia-Verdugo JM,Reiter JF

Wed Feb 20 00:00:00 UTC 2013

Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.

The EMBO journal

Kodani A,Salomé Sirerol-Piquer M,Seol A,Garcia-Verdugo JM,Reiter JF

Wed Feb 20 00:00:00 UTC 2013

Arborization of dendrites by developing neocortical neurons is dependent on primary cilia and type 3 adenylyl cyclase.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Guadiana SM,Semple-Rowland S,Daroszewski D,Madorsky I,Breunig JJ,Mykytyn K,Sarkisian MR

Wed Feb 06 00:00:00 UTC 2013

Arborization of dendrites by developing neocortical neurons is dependent on primary cilia and type 3 adenylyl cyclase.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Guadiana SM,Semple-Rowland S,Daroszewski D,Madorsky I,Breunig JJ,Mykytyn K,Sarkisian MR

Wed Feb 06 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: kinesin family protein 3A; kinesin-II subunit; Kinesin-like protein KIF3A; Microtubule plus end-directed kinesin motor 3A; N-4 kinesin

View more View less

Gene Aliases: FLA10; KIF3; KIF3A; Kifl; KLP-20; Kns3

View more View less

UniProt ID: (Human) Q9Y496, (Mouse) P28741

View more View less

Entrez Gene ID: (Human) 11127, (Mouse) 16568

View more View less

Function(s)

protein binding ATP binding microtubule binding ATP-dependent microtubule motor activity, plus-end-directed spectrin binding nucleotide binding microtubule motor activity ATPase activity protein complex binding

Process(es)

in utero embryonic development kidney development heart looping microtubule-based movement smoothened signaling pathway determination of left/right symmetry heart development epidermis development anterior/posterior pattern specification dorsal/ventral pattern formation dentate gyrus development dorsal/ventral neural tube patterning neural tube development cell projection organization nonmotile primary cilium assembly epidermal stem cell homeostasis cilium assembly positive regulation of receptor-mediated endocytosis positive regulation of epithelial cell proliferation inner ear receptor stereocilium organization cilium morphogenesis neural precursor cell proliferation motile primary cilium assembly retrograde vesicle-mediated transport, Golgi to ER organelle organization antigen processing and presentation of exogenous peptide antigen via MHC class II neurogenesis plus-end-directed vesicle transport along microtubule

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

KIF3A Polyclonal Antibody

KIF3A Antibody (13930-1-AP) in IHC (P)

Product Details

13930-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!