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KIP2 Antibody (PA5-104486) in ICC/IF
Immunocytochemistry analysis of KIP2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with KIP2 polyclonal antibody (Product # PA5-104486) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-104486
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CIB2(Accession O75838), corresponding to amino acid residues N40-D90.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2853787
Product Specific Information
Antibody detects endogenous levels of total CIB2.
Target Information
DNA-dependent protein kinases (DNA-PK) play a role in the repair of double-strand DNA breaks and in the process of V(D)J recombination during lymphoid development. By EST database searching for sequences homologous to the DNA-PK gene KIP/CIB and 5-prime RACE, Seki, N et al. isolated a full-length cDNA, which they designated as KIP2, from a human fetal brain cDNA library. KIP2 encodes a deduced 187-amino acid protein with a predicted molecular mass of 22 kD. The KIP2 protein shares 46%, 39%, and 30% sequence identity with the calcium-binding proteins KIP/CIB, calcineurin B and calmodulin respectively. KIP2 contains two EF-hand motifs and a helix-loop-helix motif involved in coordinating the calcium ion, indicating that KIP2 may also bind calcium. KIP2 is ubiquitously expressed in various human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium and integrin-binding family member 2; calcium binding protein Kip 2; calcium binding protein Kip2; DNA-dependent protein kinase catalytic subunit-interacting protein 2; kinase interacting protein 2; Kinase-interacting protein 2; KIP 2
Gene Aliases: 2810434I23Rik; AI449053; CIB2; DFNB48; KIP2; USH1J
UniProt ID: (Human) O75838, (Rat) Q568Z7, (Mouse) Q9Z309
Entrez Gene ID: (Human) 10518, (Rat) 300719, (Mouse) 56506
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