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Invitrogen
KLC1 Recombinant Rabbit Monoclonal Antibody (JE54-47)
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FIGURE: 1 / 7
KLC1 Antibody (MA5-36189) in ICC/IF
Immunocytochemical analysis of KLC1 in RWPE-1 cells using a KLC1 monoclonal antibody (Product #MA5-36189). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.
Product Details
MA5-36189
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE54-47
Immunogen
Recombinant protein within Human KLC1 aa 461-573
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2890463
Target Information
Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature of some of these variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: kinesin 2; kinesin 2 60/70kDa; Kinesin light chain 1; Kinesin2; KLC 1; KNS 2; KNS 2A; medulloblastoma antigen MU-MB-2.50; MGC15245
Gene Aliases: AI874768; KLC; KLC1; KNS2; KNS2A
UniProt ID: (Human) Q07866, (Rat) P37285
Entrez Gene ID: (Human) 3831, (Mouse) 16593, (Rat) 171041
retrograde vesicle-mediated transport, Golgi to ER
microtubule-based movement
viral process
antigen processing and presentation of exogenous peptide antigen via MHC class II
stress granule disassembly
intracellular protein transport
axo-dendritic transport
protein localization to synapse
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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