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KLF10 Antibody (PA5-89053) in WB
Western blot analysis of KLF10 in extracts of various cell lines. Samples were incubated with KLF10 Polyclonal antibody (Product # PA5-89053) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89053
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 161-365 of human KLF10 (NP_0010274531)
Conjugate
Form
Liquid
Concentration
1.15 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805324
Product Specific Information
Immunogen sequence: MKAASILNYQ NNSFRRRTHL NVEAARKNIP CAAVSPNRSK CERNTVADVD EKASAALYDF SVPSSETVIC RSQPAPVSPQ QKSVLVSPPA VSAGGVPPMP VICQMVPLPA NNPVVTTVVP STPPSQPPAV CPPVVFMGTQ VPKGAVMFVV PQPVVQSSKP PVVSPNGTRL SPIAPAPGFS PSAAKVTPQI DSSRIRSHIC SHPGC; Positive Samples: Mouse kidney, Mouse skeletal muscle, Rat kidney; Cellular Location: Nucleus
Target Information
KLF10 is a nuclear protein belonging to the Sp1 C2H2-type zinc-finger protein family with three C2H2-type zinc fingers. KLF10 is a transcriptional repressor involved in the regulation of cell growth and inhibits cell growth. It binds to the consensus sequence 5'-GGTGTG-3' and is induced by TGF-beta thus playing a role in linking TGF-beta-mediated signaling cascades to the regulation of pancreatic epithelial cell growth. KLF10 is involved in inducing and promoting apoptosis through the mitochondrial apoptotic pathway and may inhibit Smad7 expression in bronchial epithelial cells. Ubiquitous expression of KLF10 is seen in most of the tissues. Reports suggest that it may mediate up-regulation of TGFBI in VHL-deficient tumors and other cancers. Over expression of KLF10 may be associated with renal clear cell carcinoma and other tumors. KLF11 is a zinc finger transcription factor that binds to SP1-like sequences in epsilon- and gamma-globin gene promoters. This binding inhibits cell growth and causes apoptosis. Defects in this gene are a cause of maturity-onset diabetes of the young type 7 (MODY7). Three transcript variants encoding two different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GDNF-inducible factor; Krueppel-like factor 10; mGIF; TGFB inducible early growth response; TGFB inducible early growth response 1; TGFB-inducible early growth response protein 1; TIEG-1; Transcription factor GIF; Transforming growth factor-beta-inducible early growth response protein 1; Zinc finger transcription factor homolog CPG20
Gene Aliases: AI115143; Cpg20; EGR[a]; Egral; Gdnfif; Klf10; mGIF; Tieg; TIEG-1; Tieg1
UniProt ID: (Rat) O08876, (Mouse) O89091
Entrez Gene ID: (Rat) 81813, (Mouse) 21847
transcription, DNA-templated
regulation of transcription, DNA-templated
circadian rhythm
cellular response to starvation
bone mineralization
somatic stem cell population maintenance
regulation of circadian rhythm
positive regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
cellular response to peptide
rhythmic process
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