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Invitrogen
KLF14 Polyclonal Antibody
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FIGURE: 1 / 2
KLF14 Antibody (PA5-100880) in ICC/IF
Immunocytochemistry analysis of KLF14 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with KLF14 polyclonal antibody (Product # PA5-100880) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100880
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KLF14(Accession Q8TD94), corresponding to amino acid residues C260-G310.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850376
Product Specific Information
Antibody detects endogenous levels of total KLF14.
Target Information
This gene encodes a member of the Kruppel-like family of transcription factors. The gene exhibits imprinted expression from the maternal allele in embryonic and extra-embryonic tissues. Expression of this gene is induced by TGF-beta, and the protein represses TGF-beta receptor II expression. The protein functions as part of a transcriptional co-repressor complex that also contains the transcriptional regulator SIN3A and histone deacetylase 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basic transcription element-binding protein 5; BTE-binding protein 5; Krueppel-like factor 14; Kruppel-like factor 14; Transcription factor BTEB5
Gene Aliases: 5330411L03Rik; AL022736; BTEB5; KLF14
UniProt ID: (Human) Q8TD94, (Mouse) Q19A41
Entrez Gene ID: (Human) 136259, (Mouse) 619665
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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