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KLF15 Antibody (PA5-28133) in WB
Western Blot analysis of KLF15 was performed by separating 30 µg of non-transfected (-), KLF4-transfected (+) , KLF12-transfected (+) and KLF15-transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a KLF15 Polyclonal Antibody (Product # PA5-28133) at a dilution of 1:1000.
Product Details
PA5-28133
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KLF15. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2545609
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, human KLF15-transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
KLF15 is a nuclear protein belonging to the Sp1 C2H2-type zinc-finger protein family with three C2H2-type zinc fingers. A transcriptional activator, KLF15 binds to the GA element of the CLCNKA promoter and may help to regulate the strict tissue and nephron segment-specific expression of CLCNKA and CLCNKB channel genes. It plays an essential role in adipogenesis in cells through its regulation of PPARG expression. Reports suggest that KLF15 is an inhibitor of cardiac hypertrophy and functions in a pathway, regulating the cardiac response to hemodynamic stress, and regulates mitochondrial acetyl-CoA synthase expression in skeletal muscle. KLF15 may also act as a transcriptional repressor of the rhodopsin and IRBP promoters in the retina, and is a possible participant in repression of photoreceptor-specific gene expression in nonphotoreceptor cells. It is highly expressed in liver, followed by heart, skeletal muscle, and kidney but not expressed in bone marrow or lymphoid tissues.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp779M1320; Kidney-enriched krueppel-like factor; kidney-enriched Kruppel-like factor; KKLF protein; Krueppel-like factor 15; Kruppel-like factor 15
Gene Aliases: KKLF; KLF15
UniProt ID: (Human) Q9UIH9
Entrez Gene ID: (Human) 28999
transcription from RNA polymerase II promoter
glial cell differentiation
cardiac muscle hypertrophy in response to stress
glucose transport
positive regulation of transcription from RNA polymerase II promoter
glomerular visceral epithelial cell differentiation
cellular response to peptide
negative regulation of peptidyl-lysine acetylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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