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          • Primary Antibodies ›
          • KLF2 Antibodies

          Invitrogen

          KLF2 Monoclonal Antibody (OTI3A10)

          Advanced Verification
          View all (44) KLF2 antibodies

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          Datasheet
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          Cite KLF2 Monoclonal Antibody (OTI3A10)

          • Antibody Testing Data (5)
          • Advanced Verification (1)
          KLF2 Antibody in Immunocytochemistry (ICC/IF)
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          KLF2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          KLF2 Antibody (MA5-26827) in ICC/IF

          Immunofluorescence analysis of Krueppel-like factor 2 was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with KLF2 Monoclonal Antibody (OTI3A10) (Product # MA5-26827) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          KLF2 Antibody in Immunocytochemistry (ICC/IF)
          KLF2 Antibody in Immunocytochemistry (ICC/IF)
          KLF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          KLF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          KLF2 Antibody in Western Blot (WB)
          KLF2 Antibody

          Product Details

          MA5-26827

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:150
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          OTI3A10

          Immunogen

          Full length human recombinant protein of KLF2 produced in E.coli
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 1% BSA, 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2723311

          Target Information

          KLF2 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 3 C2H2-type zinc fingers. KLF2 binds to the CACCC box in the beta-globin gene promoter and activates transcription.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Krueppel-like factor 2; Kruppel-like factor 2 (lung); Kruppel-like factor LKLF; Lung krueppel-like factor; lung Kruppel-like factor; lung Kruppel-like zinc finger transcription factor

          View more View less

          Gene Aliases: KLF2; LKLF

          View more View less

          UniProt ID: (Human) Q9Y5W3

          View more View less

          Entrez Gene ID: (Human) 10365

          View more View less

          Function(s)
          transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding metal ion binding
          Process(es)
          cell morphogenesis in utero embryonic development transcription, DNA-templated negative regulation of interleukin-6 production multicellular organism growth positive regulation of transcription from RNA polymerase II promoter in response to stress regulation of gene expression, epigenetic erythrocyte maturation positive regulation of nitric oxide biosynthetic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of protein metabolic process Type I pneumocyte differentiation cellular response to hydrogen peroxide cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to cycloheximide cellular response to laminar fluid shear stress cellular response to peptide
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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