Rockland

KLF4 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-GU2

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
-

Immunohistochemistry (IHC)

1:200
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

Flow Cytometry (Flow)

Assay-dependent
-

ELISA (ELISA)

5 µg/mL
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-KLF4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Term portion of human KLF4 conjugated to Keyhole Limpet Hemocyanin (KLH).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.05 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human KLF4. Blast analysis of the sequence of the immunogen shows 100% identity with human and 93% identity to mouse.

Target Information

Kruppel like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. It is also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF) and belongs to a family of conserved Kruppel-like factor family of DNA binding zinc finger transcription factors. KLF4 exerts its functional relevance across varied cellular pathways. As one of the four Yamanaka factors, it plays a key role in reprogramming differentiated cells into pluripotent stem cells. Most studies have focused on its specific role in endothelial cell signaling, adipogenesis, differentiation, embryogenesis and cell cycle control. Localization plays an important role in the biological significance of this protein wherein nuclear localization is associated with its function as an oncogene and cytosolic localization with tumor suppression specific effect.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: endothelial Kruppel-like zinc finger protein; Epithelial zinc finger protein EZF; gut Kruppel-like factor; Gut-enriched krueppel-like factor; Krueppel-like factor 4; Kruppel-like factor 4 (Gut enriched kruppel-like factor) (Epithelial zinc-finger protein EZF); Kruppel-like factor 4 (gut); RP11-150J11.1

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Gene Aliases: EZF; GKLF; KLF4; Zie

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UniProt ID: (Human) O43474, (Mouse) Q60793

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Entrez Gene ID: (Human) 9314, (Mouse) 16600

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Function(s)
core promoter proximal region sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding transcription factor recruiting core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription cofactor binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding zinc ion binding phosphatidylinositol 3-kinase regulator activity histone deacetylase binding transcription regulatory region DNA binding nucleic acid binding DNA binding double-stranded DNA binding transcription factor binding sequence-specific DNA binding metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter negative regulation of cell proliferation epidermal cell differentiation response to organic substance negative regulation of phosphatidylinositol 3-kinase signaling negative regulation of muscle hyperplasia stem cell population maintenance cell differentiation post-embryonic camera-type eye development negative regulation of NF-kappaB transcription factor activity positive regulation of cellular protein metabolic process response to retinoic acid negative regulation of heterotypic cell-cell adhesion post-embryonic hemopoiesis regulation of cell proliferation negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of phosphatidylinositol 3-kinase activity negative regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process fat cell differentiation regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hemoglobin biosynthetic process negative regulation of smooth muscle cell proliferation epidermis morphogenesis positive regulation of protein metabolic process negative regulation of protein kinase B signaling positive regulation of telomerase activity canonical Wnt signaling pathway negative regulation of response to cytokine stimulus negative regulation of ERK1 and ERK2 cascade cellular response to growth factor stimulus cellular response to laminar fluid shear stress negative regulation of cell migration involved in sprouting angiogenesis negative regulation of chemokine (C-X-C motif) ligand 2 production mesodermal cell fate determination positive regulation of gene expression negative regulation of gene expression somatic stem cell population maintenance regulation of axon regeneration negative regulation of inflammatory response cellular response to hydrogen peroxide cellular response to retinoic acid cellular response to cycloheximide cellular response to peptide negative regulation of leukocyte adhesion to arterial endothelial cell positive regulation of transcription regulatory region DNA binding
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