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KLHL12 Antibody (MA5-49300) in IHC (P)
Immunohistochemistry analysis of KLHL12 in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with KLHL12 Monoclonal antibody (Product # MA5-49300) using a dilution of 2 μg/mL overnight at 4°C. Peroxidase Conjugated Goat Anti-mou... View More
Product Details
MA5-49300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
2G11D1
Immunogen
E.coli-derived human KLHL12 recombinant protein (Position: R27-I331).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074958
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human PC-3 whole cell, human 293T whole cell, human Hela whole cell, human Jurkat whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse RAW2647 whole cell. IHC: human liver cancer tissue, human thyroid cancer tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3.Tissue specificity: Highly expressed in testis and at lower levels in the submandibular salivary gland.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CUL3-interacting protein 1; DKIR homolog; FLJ27152; hDKIR; kelch-like 12; kelch-like family member 12; Kelch-like protein 12; kelch-like protein C3IP1
Gene Aliases: C3IP1; DKIR; KLHL12
UniProt ID: (Human) Q53G59, (Mouse) Q8BZM0, (Rat) Q8R2H4
Entrez Gene ID: (Human) 59349, (Mouse) 240756, (Rat) 266772
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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