Proteintech

KLHL22 Polyclonal Antibody

FIGURE: 1 / 3

KLHL22 Antibody (16214-1-AP) in WB

Human brain tissue were subjected to SDS PAGE followed by western blot with 16214-1-AP (KLHL22 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

KLHL22 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16214-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 9 publications 9 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLHL22 Fusion Protein Ag9212 (81-634 aa encoded by BC015923)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GGLKEMEQE EVLIHGVSYN AMCQILHFIY TSELELSLSN VQETLVAACQ LQIPEIIHFC CDFLMSWVDE ENILDVYRLA ELFDLSRLTE QLDTYILKNF VAFSRTDKYR QLPLEKVYSL LSSNRLEVSC ETEVYEGALL YHYSLEQVQA DQISLHEPPK LLETVRFPLM EAEVLQRLHD KLDPSPLRDT VASALMYHRN ESLQPSLQSP QTELRSDFQC VVGFGGIHST PSTVLSDQAK YLNPLLGEWK HFTASLAPRM SNQGIAVLNN FVYLIGGDNN VQGFRAESRC WRYDPRHNRW FQIQSLQQEH ADLSVCVVGR YIYAVAGRDY HNDLNAVERY DPATNSWAYV APLKREVYAH AGATLEGKMY ITCGRRGEDY LKETHCYDPG SNTWHTLADG PVRRAWHGMA TLLNKLYVIG GSNNDAGYRR DVHQVACYSC TSGQWSSVCP LPAGHGEPGI AVLDNRIYVL GGRSHNRGSR TGYVHIYDVE KDCWEEGPQL DNSISGLAAC VLTLPRSLLL EPPRGTPDRS QADPDFASEV MSVSDWEEFD NSSED (81-634 aa encoded by B C015923)

Target Information

KLHL22 (kelch-like protein 22) is a 634 amino acid protein that is related to the Drosophila kelch protein, which is required to maintain Actin organization in ovarian ring canals. Mutations affecting Kelch function result in the failure of Kelch to associate with the ring canals and subsequent female sterility. Human KLHL22 protein contains six kelch repeats and one BTB (POZ) domain. The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. There are two isoforms of KLHL22 that are produced as a result of alternative splicing events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Downregulation of UBE4B promotes CNS axon regrowth and functional recovery after stroke.

iScience

Jin S,Chen X,Zheng H,Cai W,Lin X,Kong X,Ni Y,Ye J,Li X,Shen L,Guo B,Abdelrahman Z,Zhou S,Mao S,Wang Y,Yao C,Gu X,Yu B,Wang Z,Wang X

Fri Jan 20 00:00:00 UTC 2023

UBAP2L-dependent coupling of PLK1 localization and stability during mitosis.

EMBO reports

Guerber L,Vuidel A,Liao Y,Kleiss C,Grandgirard E,Sumara I,Pangou E

Mon Jun 05 00:00:00 UTC 2023

XAF1 prevents hyperproduction of type I interferon upon viral infection by targeting IRF7.

EMBO reports

Liu BQ,Liu RB,Li WP,Mao XT,Li YN,Huang T,Wang HL,Chen HT,Zhong JY,Yang B,Chai RJ,Cao Q,Jin J,Li YY

Mon Jan 09 00:00:00 UTC 2023

XAF1 prevents hyperproduction of type I interferon upon viral infection by targeting IRF7.

EMBO reports

Liu BQ,Liu RB,Li WP,Mao XT,Li YN,Huang T,Wang HL,Chen HT,Zhong JY,Yang B,Chai RJ,Cao Q,Jin J,Li YY

Mon Jan 09 00:00:00 UTC 2023

Fine-tuning of mTOR signaling by the UBE4B-KLHL22 E3 ubiquitin ligase cascade in brain development.

Development (Cambridge, England)

Kong X,Shu X,Wang J,Liu D,Ni Y,Zhao W,Wang L,Gao Z,Chen J,Yang B,Guo X,Wang Z

Thu Dec 15 00:00:00 UTC 2022

A novel missense variant in CUL3 shows altered binding ability to BTB-adaptor proteins leading to diverse phenotypes of CUL3-related disorders.

Journal of human genetics

Kato K,Miya F,Oka Y,Mizuno S,Saitoh S

Sat May 01 00:00:00 UTC 2021

KLHL22 promotes malignant melanoma growth in vitro and in vivo by activating the PI3K/Akt/mTOR signaling pathway.

Neoplasma

Liu XR,Wang W,Li HM

Tue Sep 01 00:00:00 UTC 2020

KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing.

Nature

Chen J,Ou Y,Yang Y,Li W,Xu Y,Xie Y,Liu Y

Tue May 01 00:00:00 UTC 2018

KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing.

Nature

Chen J,Ou Y,Yang Y,Li W,Xu Y,Xie Y,Liu Y

Tue May 01 00:00:00 UTC 2018

SUMOylation Promotes Nuclear Import and Stabilization of Polo-like Kinase 1 to Support Its Mitotic Function.

Cell reports

Wen D,Wu J,Wang L,Fu Z

Tue Nov 21 00:00:00 UTC 2017

SUMOylation Promotes Nuclear Import and Stabilization of Polo-like Kinase 1 to Support Its Mitotic Function.

Cell reports

Wen D,Wu J,Wang L,Fu Z

Tue Nov 21 00:00:00 UTC 2017

Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis.

The Journal of cell biology

Zhuo X,Guo X,Zhang X,Jing G,Wang Y,Chen Q,Jiang Q,Liu J,Zhang C

Mon Aug 31 00:00:00 UTC 2015

Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis.

The Journal of cell biology

Zhuo X,Guo X,Zhang X,Jing G,Wang Y,Chen Q,Jiang Q,Liu J,Zhang C

Mon Aug 31 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: kelch-like 22; kelch-like family member 22; Kelch-like protein 22

View more View less

Gene Aliases: 2610318I18Rik; KELCHL; KLHL22; RGD1306288

View more View less

UniProt ID: (Human) Q53GT1, (Mouse) Q99JN2, (Rat) D3ZZC3

View more View less

Entrez Gene ID: (Human) 84861, (Mouse) 224023, (Rat) 303792

View more View less

Function(s)

ubiquitin-protein transferase activity protein binding molecular_function

Process(es)

mitotic sister chromatid segregation protein monoubiquitination mitotic spindle assembly checkpoint cell division cell cycle mitotic nuclear division protein ubiquitination

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

KLHL22 Polyclonal Antibody

KLHL22 Antibody (16214-1-AP) in WB

Product Details

16214-1-AP

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!