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Product Details
MA1-81000
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
10/78
Immunogen
Purified splenic NK cells from the LEW rat strain.
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_927712
Product Specific Information
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Rat CD161 antibody, clone 10/78 recognizes the rat Killer cell lectin-like receptor subfamily B protein, also known as NKR-PI or CD161.
Target Information
KLRB1 (Killer cell lectin like receptor subfamily B, member 1, CD-161) protein is classified as a type II membrane protein because it has an external C terminus. KLRB1 cell surface antigen is expressed by almost all NK cells and in a small subset of CD3+ve T cells. KLRB1 is a homodimeric cell surface protein, comprising two chains with molecular weights ranging from 40-44kDa. KLRB1 plays an inhibitory role on natural killer (NK) cells. Activation of KLRB1 leads to acid sphingomyelnase/SMPD1 stimulation and an increase in intracellular ceramide. Moreover, there is also an activation of AKT1/PKB and RPS6KA1/RSK1 kinase stimulation, and T cell proliferation by anti-CD3. KLRB1 acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope and to the N-acetyllactosamine epitope. KLRB1 also binds to CLEC2D/LLT1 as a ligand, and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen 3.2.3; CD161 antigen-like family member A; CD161 antigen-like family member B; CD161a; CD161b; Immunoreceptor NKR-P1E; Killer cell lectin-like receptor subfamily B member 1; Killer cell lectin-like receptor subfamily B member 1A; killer cell lectin-like receptor subfamily B member 1B; Killer cell lectin-like receptor subfamily B member 1B allele A; killer cell lectin-like receptor subfamily B member 1B allele C; killer cell lectin-like receptor subfamily B member 1F; Killer cell lectin-like receptor subfamily B member 1G; klrb1b {ECO:0000312|EMBL:AAQ11375.1}; klrb1b {ECO:0000312|RGD:2975}; MGC138614; natural killer cell receptor; Natural killer cell surface protein NKR-P1B allele RNK/SD/BN/F344; natural killer cell surface protein NKR-P1B allele TO; Natural killer cell surface protein NKR-P1G; Natural killer cell surface protein P1-3.2.3; Natural killer lectin-like receptor 1E; natural killer lectin-like receptor protein 1 e; NKR-P1 3.2.3; NKR-P1A
Gene Aliases: Klrb1; Klrb1a; Klrb1b; Klrb1d; Klrb1f; Klrb1g; Klrb6; NKR-P1A; Nkr-p1b; Nkr-p1e; NKR-P1G; Nkrp-1e; Nkrp1a; Nkrp1b; Nkrp1e; Nkrp1g
UniProt ID: (Rat) Q0ZUP0, (Rat) P27471, (Rat) A4KWA1
Entrez Gene ID: (Rat) 689817, (Rat) 362443, (Rat) 25192
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