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Invitrogen
KPNB1 Polyclonal Antibody
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FIGURE: 1 / 8
KPNB1 Antibody (PA5-18450) in ICC/IF
Immunofluorescence analysis of KPNB1 was performed using Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KPNB1 Polyclonal Antibody (Product # PA5-18450) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078) at a dilution of 1:2000 for 45 minutes at room ... View More
Product Details
PA5-18450
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (RRSKTNKAKTLAT) corresponding to the C-terminus amino acids of KPNB1 (aa 851-864).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10980020
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:6400; importin 1; importin 90; Importin beta; importin beta-1 subunit; Importin subunit beta-1; Importin-90; KapB1; karyopherin (importin) beta 1; karyopherin beta 1; Karyopherin subunit beta-1; karyopherin,beta 1; MGC2155; MGC2156; MGC2157; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97
Gene Aliases: IMB1; Impnb; IPO1; IPOB; KPNB1; NTF97
UniProt ID: (Human) Q14974, (Rat) P52296
Entrez Gene ID: (Human) 3837, (Rat) 24917
protein import into nucleus
ribosomal protein import into nucleus
intracellular protein transport
nucleocytoplasmic transport
protein import into nucleus, docking
protein import into nucleus, translocation
apoptotic DNA fragmentation
NLS-bearing protein import into nucleus
mitotic chromosome movement towards spindle pole
mitotic metaphase plate congression
modulation by virus of host process
astral microtubule organization
Ran protein signal transduction
establishment of mitotic spindle localization
establishment of protein localization
intracellular transport of virus
mitotic spindle assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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