Proteintech

KRAS-2B Polyclonal Antibody

5 References

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (14)

KRAS-2B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

KRAS-2B Antibody (16155-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

KRAS-2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KRAS-2B Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

16155-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.59 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specific for KRASB.

Target Information

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors.

Nature biotechnology

Davies CW,Oh AJ,Mroue R,Steffek M,Bruning JM,Xiao Y,Feng S,Jayakar S,Chan E,Arumugam V,Uribe SC,Drummond J,Frommlet A,Lu C,Franke Y,Merchant M,Koeppen H,Quinn JG,Malhotra S,Do S,Gazzard L,Purkey HE,Rudolph J,Mulvihill MM,Koerber JT,Wang W,Evangelista M

Sun May 01 00:00:00 UTC 2022

Targeting eIF4A-Dependent Translation of KRAS Signaling Molecules.

Cancer research

Singh K,Lin J,Lecomte N,Mohan P,Gokce A,Sanghvi VR,Jiang M,Grbovic-Huezo O,Burčul A,Stark SG,Romesser PB,Chang Q,Melchor JP,Beyer RK,Duggan M,Fukase Y,Yang G,Ouerfelli O,Viale A,de Stanchina E,Stamford AW,Meinke PT,Rätsch G,Leach SD,Ouyang Z,Wendel HG

Thu Apr 15 00:00:00 UTC 2021

Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.

Nature biomedical engineering

Labib M,Wang Z,Ahmed SU,Mohamadi RM,Duong B,Green B,Sargent EH,Kelley SO

Fri Jan 01 00:00:00 UTC 2021

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Cell chemical biology

Röth S,Macartney TJ,Konopacka A,Chan KH,Zhou H,Queisser MA,Sapkota GP

Thu Sep 17 00:00:00 UTC 2020

Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.

Science signaling

Waters AM,Ozkan-Dagliyan I,Vaseva AV,Fer N,Strathern LA,Hobbs GA,Tessier-Cloutier B,Gillette WK,Bagni R,Whiteley GR,Hartley JL,McCormick F,Cox AD,Houghton PJ,Huntsman DG,Philips MR,Der CJ

Tue Sep 26 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: c-K-ras; c-Ki-ras; c-Kirsten-ras protein; cellular c-Ki-ras2 proto-oncogene; GTPase KRas; K RAS; K-Ras 2; K-ras p21 protein; Ki-Ras; Kirsten rat sarcoma oncogene 2, expressed; Kirsten rat sarcoma viral oncogene homolog; oncogene KRAS2; p21 protein; PR310 c-K-ras oncogene; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog

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Gene Aliases: AI929937; C-K-RAS; CFC2; K-ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS; Kras-2; KRAS1; KRAS2; NS; NS3; p21B; RALD; ras; RASK2

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UniProt ID: (Human) P01116, (Mouse) P32883

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Entrez Gene ID: (Human) 3845, (Mouse) 16653

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Function(s)

GTPase activity protein binding GTP binding GMP binding GDP binding LRR domain binding protein complex binding nucleotide binding

Process(es)

MAPK cascade positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway epidermal growth factor receptor signaling pathway Ras protein signal transduction axon guidance positive regulation of cell proliferation visual learning positive regulation of gene expression cytokine-mediated signaling pathway forebrain astrocyte development actin cytoskeleton organization regulation of protein stability regulation of synaptic transmission, GABAergic positive regulation of Rac protein signal transduction social behavior endocrine signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway positive regulation of MAP kinase activity negative regulation of neuron apoptotic process negative regulation of cell differentiation regulation of long-term neuronal synaptic plasticity homeostasis of number of cells within a tissue leukocyte migration positive regulation of nitric-oxide synthase activity positive regulation of NF-kappaB transcription factor activity striated muscle cell differentiation response to glucocorticoid response to mineralocorticoid epithelial tube branching involved in lung morphogenesis signal transduction small GTPase mediated signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KRAS-2B Polyclonal Antibody

KRAS-2B Antibody (16155-1-AP) in ICC/IF

Product Details

16155-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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