Invitrogen

KSHV ORF45 Monoclonal Antibody (2D4A5)

12 Published Figures

8 References

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (12)

KSHV ORF45 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

KSHV ORF45 Antibody (MA5-14769) in ICC/IF

Immunocytochemistry analysis of TPA induced BCBL-1 cells (A) and uninduced BCBL-1 cells (B) using KSHV ORF45 monoclonal antibody (Product # MA5-14769) followed with AEC staining.

KSHV ORF45 Antibody in Western Blot (WB)

Product Image Gallery

Product Details

MA5-14769

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

1:200-1:1,000

Immunocytochemistry (ICC/IF)

Assay-dependent

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2D4A5

Immunogen

Purified recombinant fragment of KSHV ORF45 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10999794

Product Specific Information

MA5-14769 targets KSHV ORF45 in indirect ELISA, IHC and WB applications and shows reactivity with Virus samples.

The MA5-14769 immunogen is purified recombinant fragment of KSHV ORF45 expressed in E. Coli.

Target Information

Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mitochondrial protein, TBRG4, modulates KSHV and EBV reactivation from latency.

PLoS pathogens

Zhang H,Wong JP,Ni G,Cano P,Dittmer DP,Damania B

MA5-14769 was used in Western Blot to suggest that TBRG4 serves as a cellular repressor of KSHV and EBV reactivation through the regulation of ROS production.

Tue Nov 01 00:00:00 UTC 2022

Kaposi's sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesis.

Cell reports

Méndez-Solís O,Bendjennat M,Naipauer J,Theodoridis PR,Ho JJD,Verdun RE,Hare JM,Cesarman E,Lee S,Mesri EA

MA5-14769 was used in Western Blotting to identify a link between Kaposi's sarcoma herpesvirus replicative biology and oncogenicity.

Tue Dec 28 00:00:00 UTC 2021

Kaposi's sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesis.

Cell reports

Méndez-Solís O,Bendjennat M,Naipauer J,Theodoridis PR,Ho JJD,Verdun RE,Hare JM,Cesarman E,Lee S,Mesri EA

MA5-14769 was used in Western Blotting to identify a link between Kaposi's sarcoma herpesvirus replicative biology and oncogenicity.

Tue Dec 28 00:00:00 UTC 2021

PPP6C Negatively Regulates STING-Dependent Innate Immune Responses.

mBio

Ni G,Ma Z,Wong JP,Zhang Z,Cousins E,Major MB,Damania B

MA5-14769 was used in Western Blot to study the role of protein phosphatase 6 catalytic subunit in STING-dependent innate immune responses.

Tue Aug 04 00:00:00 UTC 2020

PPP6C Negatively Regulates STING-Dependent Innate Immune Responses.

mBio

Ni G,Ma Z,Wong JP,Zhang Z,Cousins E,Major MB,Damania B

MA5-14769 was used in Western Blot to study the role of protein phosphatase 6 catalytic subunit in STING-dependent innate immune responses.

Tue Aug 04 00:00:00 UTC 2020

ADAR1 Facilitates KSHV Lytic Reactivation by Modulating the RLR-Dependent Signaling Pathway.

Cell reports

Zhang H,Ni G,Damania B

MA5-14769 was used in Western Blotting to report that adenosine deaminase acting on RNA 1 (ADAR1) is required for optimal KSHV lytic reactivation from latency.

Tue Apr 28 00:00:00 UTC 2020

KSHV activates unfolded protein response sensors but suppresses downstream transcriptional responses to support lytic replication.

PLoS pathogens

Johnston BP,Pringle ES,McCormick C

Published figure using KSHV ORF45 monoclonal antibody (Product # MA5-14769) in Western Blot

Sun Dec 01 00:00:00 UTC 2019

Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication Interferes with mTORC1 Regulation of Autophagy and Viral Protein Synthesis.

Journal of virology

Pringle ES,Robinson CA,McCormick C

Published figure using KSHV ORF45 monoclonal antibody (Product # MA5-14769) in Western Blot

Fri Nov 01 00:00:00 UTC 2019

Chromatin remodeling controls Kaposi's sarcoma-associated herpesvirus reactivation from latency.

PLoS pathogens

Hopcraft SE,Pattenden SG,James LI,Frye S,Dittmer DP,Damania B

MA5-14769 was used in Western Blotting to discover new novel regulators of viral reactivation including an inhibitor of Bmi1, Romidepsin and Panobinostat.

Sat Sep 01 00:00:00 UTC 2018

NLRX1 negatively modulates type I IFN to facilitate KSHV reactivation from latency.

PLoS pathogens

Ma Z,Hopcraft SE,Yang F,Petrucelli A,Guo H,Ting JP,Dittmer DP,Damania B

Published figure using KSHV ORF45 monoclonal antibody (Product # MA5-14769) in Western Blot

Mon May 01 00:00:00 UTC 2017

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KSHV ORF45 Monoclonal Antibody (2D4A5)

KSHV ORF45 Antibody (MA5-14769) in ICC/IF

Product Details

MA5-14769

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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