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Product Details
X1255M
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
16.4
Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with cytokeratin enriched extract of feline tongue epithelium and mouse myeloma cells.
Conjugate
Form
Liquid
Concentration
0.5-2 mg/mL
Purification
Protein A/G
Storage buffer
PBS
Contains
0.08% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
This antibody detects Keratin 5 and 14 by Western blot at 58 kDa, corresponding to Keratin 5, and 50 kDa, corresponding to Keratin 14. Does not work on paraffin embedded tissue sections.
Target Information
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 58 kDa cytokeratin; CK-14; CK-5; cytokeratin 14; cytokeratin 5; Cytokeratin-14; Cytokeratin-5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; epidermolysis bullosa simplex, Dowling-Meara, Koebner; K14; K5; keratin 14, type I; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin complex 1, acidic, gene 14; keratin complex 2, basic, gene 5; Keratin, type I cytoskeletal 14; Keratin, type II cytoskeletal 5; Keratin-14; Keratin-5; krt5; tuftelin interacting protein 8; Type-II keratin Kb5
Gene Aliases: 3300001P10Rik; AI626930; AW146334; CK14; CK5; DDD; DDD1; EBS2; EBS3; EBS4; K14; K5; Krt-1.14; Krt1-14; KRT14; Krt2-5; KRT5; KRT5A; NFJ; Tfip8
UniProt ID: (Human) P02533, (Human) P13647, (Mouse) Q61781, (Mouse) Q922U2
Entrez Gene ID: (Human) 3861, (Human) 3852, (Mouse) 16664, (Mouse) 110308
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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