Invitrogen

Ki-67 Monoclonal Antibody (20Raj1), eBioscience™

Ki-67 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 24

Ki-67 Antibody (14-5699-82) in ICC/IF

Immunofluorescence analysis of Ki67 was performed using 70% confluent log phase HeLa cells serum starved for 36 Hrs followed by serum release for 6 Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Ki-67 Monoclonal Antibody (20Raj1), eBioscience™ (Product # 14-5699-82) at 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Supe... View More

Ki-67 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ki-67 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Ki-67 Antibody — Immunofluorescence analysis of Ki67 was performed using 70% confluent log phase HeLa cells serum starved for 36 Hrs followed by serum release for 6 Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Ki-67 Monoclonal Antibody (20Raj1), eBioscience™ (Product # 14-5699-82) at 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2,000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415 , 1:300). Panel d represents the merged image showing Cytoplasmic localization. Panel e represents HeLa cells serum starved for 36 Hrs with reduced signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. {TM}

Product Details

14-5699-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:500-1:1,000

View 3 publications 3 publications

Flow Cytometry (Flow)

View 15 publications 15 publications

Product Specifications

Species Reactivity

Dog, Human

Published species

Dog, Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

20Raj1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2016711

Product Specific Information

Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells.

This monoclonal antibody 20Raj1 recognizes canine (dog) Ki-67.

Applications Reported: This 20Raj1 antibody has been reported for use in immunocytochemistry and immunohistochemistry on formalin-fixed paraffin embedded tissues.

Applications Tested: This 20Raj1 antibody has been tested by immunocytochemistry on paraformaldehyde fixed and permeabilized MCF7, MDCK, and MDA-MB-175 cell lines.

This 20Raj1 antibody has been tested by immunohistochemistry on FFPE human skin using citrate buffer antigen retrieval. For immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 0.5-1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Correlation between E-cadherin expression and tumor grade, proliferation, microvascular density, and apoptosis in canine cutaneous squamous cell carcinoma.

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire

Gupta A,Al-Dissi A

14-5699-82 was used in Immunohistochemistry to examine the expression of E-cadherin in canine cutaneous squamous cell carcinoma (SCC) and determined its association with tumor grade (TG), proliferation index (PI), apoptosis index (AI), and intra-tumoral microvascular density (iMVD) in archived samples.

Sun Jan 01 00:00:00 UTC 2023

Eukaryotic initiation factor 3b regulates the development and progression of breast cancer.

American journal of cancer research

Li Y,Zhao J,Yuan Z,Yuan D,Fang M,Xie M,Xia M

14-5699-82 was used in Western Blot to examine the expression of eIF3b and found an upregulation of eIF3b in breast cancer cell lines as well as tumor tissues.

Mon Jun 12 00:00:00 UTC 2023

GABP couples oncogene signaling to telomere regulation in TERT promoter mutant cancer.

Cell reports

McKinney AM,Mathur R,Stevers NO,Molinaro AM,Chang SM,Phillips JJ,Costello JF

14-5699-82 was used in Flow Cytometry to study whether the role of epidermal growth factor receptor (EGFR) amplification to drive proliferation and telomerase reverse transcriptase promoter (TERTp) mutations underlying telomerase reactivation through recruitment of GA-binding protein (GABP) are functionally connected in cancer.

Tue Sep 20 00:00:00 UTC 2022

Acetyl-CoA Synthetase 2: A Critical Linkage in Obesity-Induced Tumorigenesis in Myeloma.

Cell metabolism

Li Z,Liu H,He J,Wang Z,Yin Z,You G,Wang Z,Davis RE,Lin P,Bergsagel PL,Manasanch EE,Wong STC,Esnaola NF,Chang JC,Orlowski RZ,Yi Q,Yang J

14-5699-82 was used in Immunohistochemistry to demonstrate a key impact for obesity-induced acetyl-CoA synthetase 2 on the progression of myeloma.

Tue Jan 05 00:00:00 UTC 2021

Protein/AS01_B vaccination elicits stronger, more Th2-skewed antigen-specific human T follicular helper cell responses than heterologous viral vectors.

Cell reports. Medicine

Nielsen CM,Ogbe A,Pedroza-Pacheco I,Doeleman SE,Chen Y,Silk SE,Barrett JR,Elias SC,Miura K,Diouf A,Bardelli M,Dabbs RA,Barfod L,Long CA,Haynes BF,Payne RO,Minassian AM,Bradley T,Draper SJ,Borrow P

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Flow Cytometry

Tue Mar 16 00:00:00 UTC 2021

Microbial exposure during early human development primes fetal immune cells.

Cell

Mishra A,Lai GC,Yao LJ,Aung TT,Shental N,Rotter-Maskowitz A,Shepherdson E,Singh GSN,Pai R,Shanti A,Wong RMM,Lee A,Khyriem C,Dutertre CA,Chakarov S,Srinivasan KG,Shadan NB,Zhang XM,Khalilnezhad S,Cottier F,Tan ASM,Low G,Chen P,Fan Y,Hor PX,Lee AKM,Choolani M,Vermijlen D,Sharma A,Fuks G,Straussman R,Pavelka N,Malleret B,McGovern N,Albani S,Chan JKY,Ginhoux F

14-5699-82 was used in Immunocytochemistry, Immunohistochemistry (Paraffin) to indicate selective presence of live microbes in fetal organs during the 2nd trimester of gestation and have broader implications toward the establishment of immune competency and priming before birth.

Thu Jun 24 00:00:00 UTC 2021

Effects of Tyrosine Kinase Inhibitor-masitinib Mesylate on Canine Mammary Tumour Cell Lines.

Journal of veterinary research

Ustun-Alkan F,Bakırel T,Üstüner O,Anlas C,Cinar S,Yıldırım F,Gürel A

14-5699-82 was used in Immunocytochemistry, Immunohistochemistry (Paraffin) to investigate the antiproliferative effect of masitinib in CMT cells and its possible mechanisms of action.

Wed Sep 01 00:00:00 UTC 2021

T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer.

Oncoimmunology

O'Connor RA,Chauhan V,Mathieson L,Titmarsh H,Koppensteiner L,Young I,Tagliavini G,Dorward DA,Prost S,Dhaliwal K,Wallace WA,Akram AR

14-5699-82 was used in Flow Cytometry to study co-inhibitory ligands in T cell immunosuppression in non-small cell lung cancer.

Mon Aug 02 00:00:00 UTC 2021

Circulating Tumor Cells Exhibit Metastatic Tropism and Reveal Brain Metastasis Drivers.

Cancer discovery

Klotz R,Thomas A,Teng T,Han SM,Iriondo O,Li L,Restrepo-Vassalli S,Wang A,Izadian N,MacKay M,Moon BS,Liu KJ,Ganesan SK,Lee G,Kang DS,Walmsley CS,Pinto C,Press MF,Lu W,Lu J,Juric D,Bardia A,Hicks J,Salhia B,Attenello F,Smith AD,Yu M

14-5699-82 was used in Immunohistochemistry (Paraffin) to demonstrate that ex vivo-cultured CTCs from 4 patients with breast cancer showed organotropism, revealing molecular features that allow a subset of CTCs to enter and grow in the brain.

Wed Jan 01 00:00:00 UTC 2020

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

The Journal of clinical investigation

Guedan S,Madar A,Casado-Medrano V,Shaw C,Wing A,Liu F,Young RM,June CH,Posey AD

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Flow Cytometry

Mon Jun 01 00:00:00 UTC 2020

Human Liver Memory CD8⁺ T Cells Use Autophagy for Tissue Residence.

Cell reports

Swadling L,Pallett LJ,Diniz MO,Baker JM,Amin OE,Stegmann KA,Burton AR,Schmidt NM,Jeffery-Smith A,Zakeri N,Suveizdyte K,Froghi F,Fusai G,Rosenberg WM,Davidson BR,Schurich A,Simon AK,Maini MK

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Flow Cytometry

Tue Jan 21 00:00:00 UTC 2020

Functional Study of the Retrotransposon-Derived Human PEG10 Protease.

International journal of molecular sciences

Golda M,Mótyán JA,Mahdi M,Tőzsér J

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Flow Cytometry

Tue Mar 31 00:00:00 UTC 2020

Niraparib activates interferon signaling and potentiates anti-PD-1 antibody efficacy in tumor models.

Scientific reports

Wang Z,Sun K,Xiao Y,Feng B,Mikule K,Ma X,Feng N,Vellano CP,Federico L,Marszalek JR,Mills GB,Hanke J,Ramaswamy S,Wang J

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Flow Cytometry

Tue Feb 12 00:00:00 UTC 2019

Tissue-resident memory T cells are epigenetically cytotoxic with signs of exhaustion in human urinary bladder cancer.

Clinical and experimental immunology

Hartana CA,Ahlén Bergman E,Broomé A,Berglund S,Johansson M,Alamdari F,Jakubczyk T,Huge Y,Aljabery F,Palmqvist K,Holmström B,Glise H,Riklund K,Sherif A,Winqvist O

14-5699 was used in Flow cytometry/Cell sorting to identify TRM cells as potential new targets for cancer immunotherapy.

Mon Oct 01 00:00:00 UTC 2018

Generation of Intestinal Organoids Suitable for Pharmacokinetic Studies from Human Induced Pluripotent Stem Cells.

Drug metabolism and disposition: the biological fate of chemicals

Onozato D,Yamashita M,Nakanishi A,Akagawa T,Kida Y,Ogawa I,Hashita T,Iwao T,Matsunaga T

14-5699-82 was used in Immunohistochemistry to demonstrate the successful generation of pharmacokinetically functional intestinal organoids from human iPS cells.

Thu Nov 01 00:00:00 UTC 2018

Involvement of TWEAK and the NF-κB signaling pathway in lupus nephritis.

Experimental and therapeutic medicine

Sun F,Teng J,Yu P,Li W,Chang J,Xu H

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Flow Cytometry

Thu Mar 01 00:00:00 UTC 2018

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

eLife

Lin JR,Izar B,Wang S,Yapp C,Mei S,Shah PM,Santagata S,Sorger PK

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Flow Cytometry

Wed Jul 11 00:00:00 UTC 2018

Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency.

Scientific reports

Nguyen HT,Noguchi S,Sugie K,Matsuo Y,Nguyen CTH,Koito H,Shiojima I,Nishino I,Tsukaguchi H

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Flow Cytometry

Tue Feb 20 00:00:00 UTC 2018

Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction.

Advanced pharmaceutical bulletin

Faramarzian Azimi Maragheh B,Fatourachi P,Mohammadi SM,Valipour B,Behtari M,Dehnad A,Nozad Charoudeh H

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Flow Cytometry

Thu Nov 01 00:00:00 UTC 2018

Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage.

Scientific reports

Christoforou N,Chakraborty S,Kirkton RD,Adler AF,Addis RC,Leong KW

14569982 was used in immunocytochemistry to describe the transdifferentiation of human dermal fibroblasts towards the cardiac cell lineage

Tue Jan 10 00:00:00 UTC 2017

Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage.

Scientific reports

Christoforou N,Chakraborty S,Kirkton RD,Adler AF,Addis RC,Leong KW

14569982 was used in immunocytochemistry to describe the transdifferentiation of human dermal fibroblasts towards the cardiac cell lineage

Tue Jan 10 00:00:00 UTC 2017

S100A6 Regulates Endothelial Cell Cycle Progression by Attenuating Antiproliferative Signal Transducers and Activators of Transcription 1 Signaling.

Arteriosclerosis, thrombosis, and vascular biology

Lerchenmüller C,Heißenberg J,Damilano F,Bezzeridis VJ,Krämer I,Bochaton-Piallat ML,Hirschberg K,Busch M,Katus HA,Peppel K,Rosenzweig A,Busch H,Boerries M,Most P

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Immunocytochemistry

Thu Sep 01 00:00:00 UTC 2016

S100A6 Regulates Endothelial Cell Cycle Progression by Attenuating Antiproliferative Signal Transducers and Activators of Transcription 1 Signaling.

Arteriosclerosis, thrombosis, and vascular biology

Lerchenmüller C,Heißenberg J,Damilano F,Bezzeridis VJ,Krämer I,Bochaton-Piallat ML,Hirschberg K,Busch M,Katus HA,Peppel K,Rosenzweig A,Busch H,Boerries M,Most P

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Immunocytochemistry

Thu Sep 01 00:00:00 UTC 2016

Interleukin-7 and Toll-like receptor 7 induce synergistic B cell and T cell activation.

PloS one

Bikker A,Kruize AA,van der Wurff-Jacobs KM,Peters RP,Kleinjan M,Redegeld F,de Jager W,Lafeber FP,van Roon JA

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Flow Cytometry

Sat Feb 07 00:00:00 UTC 2015

Somatostatin expression in human hair follicles and its potential role in immune privilege.

The Journal of investigative dermatology

Breitkopf T,Lo BK,Leung G,Wang E,Yu M,Carr N,Zloty D,Cowan B,Shapiro J,McElwee KJ

14-5699 was used in Flow cytometry/Cell sorting to demonstrate and quantify the functional immune privilege properties apparent in human hair follicles.

Mon Jul 01 00:00:00 UTC 2013

Biliary tree stem cells, precursors to pancreatic committed progenitors: evidence for possible life-long pancreatic organogenesis.

Stem cells (Dayton, Ohio)

Wang Y,Lanzoni G,Carpino G,Cui CB,Dominguez-Bendala J,Wauthier E,Cardinale V,Oikawa T,Pileggi A,Gerber D,Furth ME,Alvaro D,Gaudio E,Inverardi L,Reid LM

Published figure using Ki-67 monoclonal antibody (Product # 14-5699-82) in Immunofluorescence

Sun Sep 01 00:00:00 UTC 2013

No evidence for neo-oogenesis may link to ovarian senescence in adult monkey.

Stem cells (Dayton, Ohio)

Yuan J,Zhang D,Wang L,Liu M,Mao J,Yin Y,Ye X,Liu N,Han J,Gao Y,Cheng T,Keefe DL,Liu L

14-5699-82 was used in Immunohistochemistry to support the paradigm that adult ovaries do not undergo germ cell renewal, which may contribute significantly to ovarian senescence that occurs with age.

Fri Nov 01 00:00:00 UTC 2013

Dll4-Notch signaling in Flt3-independent dendritic cell development and autoimmunity in mice.

The Journal of experimental medicine

Billiard F,Lobry C,Darrasse-Jèze G,Waite J,Liu X,Mouquet H,DaNave A,Tait M,Idoyaga J,Leboeuf M,Kyratsous CA,Burton J,Kalter J,Klinakis A,Zhang W,Thurston G,Merad M,Steinman RM,Murphy AJ,Yancopoulos GD,Aifantis I,Skokos D

14-5699 was used in Flow cytometry/Cell sorting to investigate how Dll4-Notch signaling affects pro-T cell fate and thymic DC development, showing that it is a novel Flt3-independent pathway for DC development and autoimmunity.

Mon May 07 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2

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Gene Aliases: KIA; MIB-; MIB-1; MKI67; PPP1R105

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UniProt ID: (Human) P46013

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Entrez Gene ID: (Dog) 100686578, (Human) 4288

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Function(s)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding

Process(es)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ki-67 Monoclonal Antibody (20Raj1), eBioscience™

Ki-67 Antibody (14-5699-82) in ICC/IF

Product Details

14-5699-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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