Proteintech

Kindlin 2 Polyclonal Antibody

Kindlin 2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

Kindlin 2 Antibody (11453-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using FERMT2 antibody 11453-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Kindlin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Kindlin 2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11453-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Hamster, Human, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Kindlin 2 Fusion Protein Ag2013 (331-680 aa encoded by BC017327)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

11453-1-AP is a rabbit polyclonal antibody against human FERMT2.

Immunogen sequence: TSENHLNNS DKEVDEVDAA LSDLEITLEG GKTSTILGDI TSIPELADYI KVFKPKKLTL KGYKQYWCTF KDTSISCYKS KEESSGTPAH QMNLRGCEVT PDVNISGQKF NIKLLIPVAE GMNEIWLRCD NEKQYAHWMA ACRLASKGKT MADSSYNLEV QNILSFLKMQ HLNPDPQLIP EQITTDITPE CLVSPRYLKK YKNKQITARI LEAHQNVAQM SLIEAKMRFI QAWQSLPEFG ITHFIARFQG GKKEELIGIA YNRLIRMDAS TGDAIKTWRF SNMKQWNVNW EIKMVTVEFA DEVRLSFICT EVDCKVVHEF IGGYIFLSTR AKDQNESLDE EMFYKLTSGW V (331-680 aa encoded by BC017327)

Target Information

Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Distinct bidirectional regulation of LFA1 and α4β7 by Rap1 and integrin adaptors in T cells under shear flow.

Cell reports

Kamioka Y,Ueda Y,Kondo N,Tokuhiro K,Ikeda Y,Bergmeier W,Kinashi T

Tue Jun 27 00:00:00 UTC 2023

Kindlin-2 promotes Src-mediated tyrosine phosphorylation of androgen receptor and contributes to breast cancer progression.

Cell death & disease

Ma L,Tian Y,Qian T,Li W,Liu C,Chu B,Kong Q,Cai R,Bai P,Ma L,Deng Y,Tian R,Wu C,Sun Y

Fri May 20 00:00:00 UTC 2022

Kindlin-2 promotes Src-mediated tyrosine phosphorylation of androgen receptor and contributes to breast cancer progression.

Cell death & disease

Ma L,Tian Y,Qian T,Li W,Liu C,Chu B,Kong Q,Cai R,Bai P,Ma L,Deng Y,Tian R,Wu C,Sun Y

Fri May 20 00:00:00 UTC 2022

Kindlin-2 promotes Src-mediated tyrosine phosphorylation of androgen receptor and contributes to breast cancer progression.

Cell death & disease

Ma L,Tian Y,Qian T,Li W,Liu C,Chu B,Kong Q,Cai R,Bai P,Ma L,Deng Y,Tian R,Wu C,Sun Y

Fri May 20 00:00:00 UTC 2022

Kindlin-2 promotes Src-mediated tyrosine phosphorylation of androgen receptor and contributes to breast cancer progression.

Cell death & disease

Ma L,Tian Y,Qian T,Li W,Liu C,Chu B,Kong Q,Cai R,Bai P,Ma L,Deng Y,Tian R,Wu C,Sun Y

Fri May 20 00:00:00 UTC 2022

Molecular basis of astrocyte diversity and morphology across the CNS in health and disease.

Science (New York, N.Y.)

Endo F,Kasai A,Soto JS,Yu X,Qu Z,Hashimoto H,Gradinaru V,Kawaguchi R,Khakh BS

Fri Nov 04 00:00:00 UTC 2022

Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition.

Cell reports

Hadjisavva R,Anastasiou O,Ioannou PS,Zheltkova M,Skourides PA

Tue Jul 19 00:00:00 UTC 2022

Kindlin-2 promotes rear focal adhesion disassembly and directional persistence during cell migration.

Journal of cell science

Liu J,Liu Z,Chen K,Chen W,Fang X,Li M,Zhou X,Ding N,Lei H,Guo C,Qian T,Wang Y,Liu L,Chen Y,Zhao H,Sun Y,Deng Y,Wu C

Wed Jan 13 00:00:00 UTC 2021

Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma.

BMC cancer

Zhai Y,Zhao B,Wang Y,Li L,Li J,Li X,Chang L,Chen Q,Liao Z

Mon Mar 01 00:00:00 UTC 2021

Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression.

American journal of respiratory cell and molecular biology

Zhang P,Wang J,Luo W,Yuan J,Cui C,Guo L,Wu C

Thu Jul 01 00:00:00 UTC 2021

Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression.

American journal of respiratory cell and molecular biology

Zhang P,Wang J,Luo W,Yuan J,Cui C,Guo L,Wu C

Thu Jul 01 00:00:00 UTC 2021

Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression.

American journal of respiratory cell and molecular biology

Zhang P,Wang J,Luo W,Yuan J,Cui C,Guo L,Wu C

Thu Jul 01 00:00:00 UTC 2021

Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression.

American journal of respiratory cell and molecular biology

Zhang P,Wang J,Luo W,Yuan J,Cui C,Guo L,Wu C

Thu Jul 01 00:00:00 UTC 2021

Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression.

American journal of respiratory cell and molecular biology

Zhang P,Wang J,Luo W,Yuan J,Cui C,Guo L,Wu C

Thu Jul 01 00:00:00 UTC 2021

Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression.

American journal of respiratory cell and molecular biology

Zhang P,Wang J,Luo W,Yuan J,Cui C,Guo L,Wu C

Thu Jul 01 00:00:00 UTC 2021

Kindlin‑2 suppresses cervical cancer cell migration through AKT/mTOR‑mediated autophagy induction.

Oncology reports

Wu G,Long Y,Lu Y,Feng Y,Yang X,Xu X,Yao D

Wed Jul 01 00:00:00 UTC 2020

Kindlin‑2 suppresses cervical cancer cell migration through AKT/mTOR‑mediated autophagy induction.

Oncology reports

Wu G,Long Y,Lu Y,Feng Y,Yang X,Xu X,Yao D

Wed Jul 01 00:00:00 UTC 2020

PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.

Nature communications

Guo L,Cui C,Wang J,Yuan J,Yang Q,Zhang P,Su W,Bao R,Ran J,Wu C

Thu Oct 01 00:00:00 UTC 2020

PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.

Nature communications

Guo L,Cui C,Wang J,Yuan J,Yang Q,Zhang P,Su W,Bao R,Ran J,Wu C

Thu Oct 01 00:00:00 UTC 2020

Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.

Stem cell research & therapy

Leng L,Ma J,Lv L,Wang W,Gao D,Zhu Y,Wu Z

Wed Sep 23 00:00:00 UTC 2020

Kindlin-2 links mechano-environment to proline synthesis and tumor growth.

Nature communications

Guo L,Cui C,Zhang K,Wang J,Wang Y,Lu Y,Chen K,Yuan J,Xiao G,Tang B,Sun Y,Wu C

Tue Feb 19 00:00:00 UTC 2019

Kindlin-2 interacts with a highly conserved surface of ILK to regulate focal adhesion localization and cell spreading.

Journal of cell science

Kadry YA,Huet-Calderwood C,Simon B,Calderwood DA

Fri Oct 26 00:00:00 UTC 2018

Kindlin-2 interacts with a highly conserved surface of ILK to regulate focal adhesion localization and cell spreading.

Journal of cell science

Kadry YA,Huet-Calderwood C,Simon B,Calderwood DA

Fri Oct 26 00:00:00 UTC 2018

Kindlin-2 regulates hepatic stellate cells activation and liver fibrogenesis.

Cell death discovery

Yu J,Hu Y,Gao Y,Li Q,Zeng Z,Li Y,Chen H

Thu Oct 01 00:00:00 UTC 2020

Kindlin-2 regulates hepatic stellate cells activation and liver fibrogenesis.

Cell death discovery

Yu J,Hu Y,Gao Y,Li Q,Zeng Z,Li Y,Chen H

Thu Oct 01 00:00:00 UTC 2020

Kindlin-2 regulates hepatic stellate cells activation and liver fibrogenesis.

Cell death discovery

Yu J,Hu Y,Gao Y,Li Q,Zeng Z,Li Y,Chen H

Thu Oct 01 00:00:00 UTC 2020

Kindlin-2 regulates hepatic stellate cells activation and liver fibrogenesis.

Cell death discovery

Yu J,Hu Y,Gao Y,Li Q,Zeng Z,Li Y,Chen H

Thu Oct 01 00:00:00 UTC 2020

Kindlin-2 regulates hepatic stellate cells activation and liver fibrogenesis.

Cell death discovery

Yu J,Hu Y,Gao Y,Li Q,Zeng Z,Li Y,Chen H

Thu Oct 01 00:00:00 UTC 2020

Kindlin-2 regulates hepatic stellate cells activation and liver fibrogenesis.

Cell death discovery

Yu J,Hu Y,Gao Y,Li Q,Zeng Z,Li Y,Chen H

Thu Oct 01 00:00:00 UTC 2020

Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.

Journal of the American Society of Nephrology : JASN

Sun Y,Guo C,Ma P,Lai Y,Yang F,Cai J,Cheng Z,Zhang K,Liu Z,Tian Y,Sheng Y,Tian R,Deng Y,Xiao G,Wu C

Fri Dec 01 00:00:00 UTC 2017

Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.

Journal of the American Society of Nephrology : JASN

Sun Y,Guo C,Ma P,Lai Y,Yang F,Cai J,Cheng Z,Zhang K,Liu Z,Tian Y,Sheng Y,Tian R,Deng Y,Xiao G,Wu C

Fri Dec 01 00:00:00 UTC 2017

Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.

Journal of the American Society of Nephrology : JASN

Sun Y,Guo C,Ma P,Lai Y,Yang F,Cai J,Cheng Z,Zhang K,Liu Z,Tian Y,Sheng Y,Tian R,Deng Y,Xiao G,Wu C

Fri Dec 01 00:00:00 UTC 2017

Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.

Journal of the American Society of Nephrology : JASN

Sun Y,Guo C,Ma P,Lai Y,Yang F,Cai J,Cheng Z,Zhang K,Liu Z,Tian Y,Sheng Y,Tian R,Deng Y,Xiao G,Wu C

Fri Dec 01 00:00:00 UTC 2017

Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.

Journal of cell science

Huet-Calderwood C,Brahme NN,Kumar N,Stiegler AL,Raghavan S,Boggon TJ,Calderwood DA

Wed Oct 01 00:00:00 UTC 2014

Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.

Journal of cell science

Huet-Calderwood C,Brahme NN,Kumar N,Stiegler AL,Raghavan S,Boggon TJ,Calderwood DA

Wed Oct 01 00:00:00 UTC 2014

Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.

Journal of cell science

Huet-Calderwood C,Brahme NN,Kumar N,Stiegler AL,Raghavan S,Boggon TJ,Calderwood DA

Wed Oct 01 00:00:00 UTC 2014

Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.

Journal of cell science

Huet-Calderwood C,Brahme NN,Kumar N,Stiegler AL,Raghavan S,Boggon TJ,Calderwood DA

Wed Oct 01 00:00:00 UTC 2014

Kindlin-2 expression in peritumoral stroma is associated with poor prognosis in pancreatic ductal adenocarcinoma.

Pancreas

Mahawithitwong P,Ohuchida K,Ikenaga N,Fujita H,Zhao M,Kozono S,Shindo K,Ohtsuka T,Mizumoto K,Tanaka M

Wed May 01 00:00:00 UTC 2013

Kindlin-2 expression in peritumoral stroma is associated with poor prognosis in pancreatic ductal adenocarcinoma.

Pancreas

Mahawithitwong P,Ohuchida K,Ikenaga N,Fujita H,Zhao M,Kozono S,Shindo K,Ohtsuka T,Mizumoto K,Tanaka M

Wed May 01 00:00:00 UTC 2013

Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics.

The Journal of biological chemistry

Brahme NN,Harburger DS,Kemp-O'Brien K,Stewart R,Raghavan S,Parsons M,Calderwood DA

Fri Dec 06 00:00:00 UTC 2013

Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics.

The Journal of biological chemistry

Brahme NN,Harburger DS,Kemp-O'Brien K,Stewart R,Raghavan S,Parsons M,Calderwood DA

Fri Dec 06 00:00:00 UTC 2013

Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics.

The Journal of biological chemistry

Brahme NN,Harburger DS,Kemp-O'Brien K,Stewart R,Raghavan S,Parsons M,Calderwood DA

Fri Dec 06 00:00:00 UTC 2013

Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics.

The Journal of biological chemistry

Brahme NN,Harburger DS,Kemp-O'Brien K,Stewart R,Raghavan S,Parsons M,Calderwood DA

Fri Dec 06 00:00:00 UTC 2013

Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics.

The Journal of biological chemistry

Brahme NN,Harburger DS,Kemp-O'Brien K,Stewart R,Raghavan S,Parsons M,Calderwood DA

Fri Dec 06 00:00:00 UTC 2013

Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics.

The Journal of biological chemistry

Brahme NN,Harburger DS,Kemp-O'Brien K,Stewart R,Raghavan S,Parsons M,Calderwood DA

Fri Dec 06 00:00:00 UTC 2013

The integrin-linked kinase-PINCH-parvin complex supports integrin αIIbβ3 activation.

PloS one

Honda S,Shirotani-Ikejima H,Tadokoro S,Tomiyama Y,Miyata T

Mon Oct 13 00:00:00 UTC 2014

The integrin-linked kinase-PINCH-parvin complex supports integrin αIIbβ3 activation.

PloS one

Honda S,Shirotani-Ikejima H,Tadokoro S,Tomiyama Y,Miyata T

Mon Oct 13 00:00:00 UTC 2014

Comparative proteomic analysis of primary schwann cells and a spontaneously immortalized schwann cell line RSC 96: a comprehensive overview with a focus on cell adhesion and migration related proteins.

Journal of proteome research

Ji Y,Shen M,Wang X,Zhang S,Yu S,Chen G,Gu X,Ding F

Fri Jun 01 00:00:00 UTC 2012

Journal of proteome research

Ji Y,Shen M,Wang X,Zhang S,Yu S,Chen G,Gu X,Ding F

Fri Jun 01 00:00:00 UTC 2012

Kindlin-3 mediates integrin αLβ2 outside-in signaling, and it interacts with scaffold protein receptor for activated-C kinase 1 (RACK1).

The Journal of biological chemistry

Feng C,Li YF,Yau YH,Lee HS,Tang XY,Xue ZH,Zhou YC,Lim WM,Cornvik TC,Ruedl C,Shochat SG,Tan SM

Fri Mar 30 00:00:00 UTC 2012

Kindlin-3 mediates integrin αLβ2 outside-in signaling, and it interacts with scaffold protein receptor for activated-C kinase 1 (RACK1).

The Journal of biological chemistry

Feng C,Li YF,Yau YH,Lee HS,Tang XY,Xue ZH,Zhou YC,Lim WM,Cornvik TC,Ruedl C,Shochat SG,Tan SM

Fri Mar 30 00:00:00 UTC 2012

Kindlin-2 expression in arsenite- and cadmium-transformed bladder cancer cell lines and in archival specimens of human bladder cancer.

Urology

Talaat S,Somji S,Toni C,Garrett SH,Zhou XD,Sens MA,Sens DA

Wed Jun 01 00:00:00 UTC 2011

Kindlin-2 expression in arsenite- and cadmium-transformed bladder cancer cell lines and in archival specimens of human bladder cancer.

Urology

Talaat S,Somji S,Toni C,Garrett SH,Zhou XD,Sens MA,Sens DA

Wed Jun 01 00:00:00 UTC 2011

Kindlin-2 expression in arsenite- and cadmium-transformed bladder cancer cell lines and in archival specimens of human bladder cancer.

Urology

Talaat S,Somji S,Toni C,Garrett SH,Zhou XD,Sens MA,Sens DA

Wed Jun 01 00:00:00 UTC 2011

Kindlin-2 expression in arsenite- and cadmium-transformed bladder cancer cell lines and in archival specimens of human bladder cancer.

Urology

Talaat S,Somji S,Toni C,Garrett SH,Zhou XD,Sens MA,Sens DA

Wed Jun 01 00:00:00 UTC 2011

Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.

Circulation research

Dowling JJ,Gibbs E,Russell M,Goldman D,Minarcik J,Golden JA,Feldman EL

Fri Feb 29 00:00:00 UTC 2008

Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.

Circulation research

Dowling JJ,Gibbs E,Russell M,Goldman D,Minarcik J,Golden JA,Feldman EL

Fri Feb 29 00:00:00 UTC 2008

Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.

Circulation research

Dowling JJ,Gibbs E,Russell M,Goldman D,Minarcik J,Golden JA,Feldman EL

Fri Feb 29 00:00:00 UTC 2008

Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.

Circulation research

Dowling JJ,Gibbs E,Russell M,Goldman D,Minarcik J,Golden JA,Feldman EL

Fri Feb 29 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: Fermitin family homolog 2; kindlin 2; Kindlin-2; MIG-2; mitogen inducible 2; mitogen inducible gene 2 protein; Mitogen-inducible gene 2 protein; PH domain-containing family C member 1; pleckstrin homology domain containing, family C (with FERM domain) member 1; pleckstrin homology domain containing, family C member 1; Pleckstrin homology domain-containing family C member 1

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Gene Aliases: AA960555; FERMT2; KIND2; Kindlin-2; mig-2; MIG2; PLEKHC1; UNC112; UNC112B

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UniProt ID: (Human) Q96AC1, (Mouse) Q8CIB5

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Entrez Gene ID: (Human) 10979, (Mouse) 218952, (Rat) 289992

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Function(s)

protein binding phosphatidylinositol-3,4,5-trisphosphate binding lipid binding

Process(es)

cell adhesion cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway regulation of cell shape Wnt signaling pathway integrin activation substrate adhesion-dependent cell spreading focal adhesion assembly protein localization to membrane cell junction assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Kindlin 2 Polyclonal Antibody

Kindlin 2 Antibody (11453-1-AP) in ICC/IF

Product Details

11453-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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