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Invitrogen
Kinesin 5A Polyclonal Antibody
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FIGURE: 1 / 5
Kinesin 5A Antibody (PA1-642) in ICC/IF
Immunofluorescence analysis of Kinesin 5A was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Kinesin 5A Rabbit Polyclonal Antibody (Product # PA1-642) at 1 µg/mL in 1% BSA and incubated for 3 hours at room tem... View More
Product Details
PA1-642
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C(1007) G Y E A E D Q A K L F P L H Q E T A A S(1027)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2132216
Product Specific Information
PA1-642 detects kinesin 5A from mouse, human, and bovine samples. This antibody is specific for kinesin 5A and does not detect other kinesin isotypes.
PA1-642 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing kinesin 5A from mouse neuronal cell lysate and human retinal extracts.
The PA1-642 immunizing peptide corresponds to amino acid residues 1007-1027 from mouse kinesin 5A. This sequence is completely conserved in human. This peptide (Cat. # PEP-197) is available for use in neutralization and control experiments.
Target Information
This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: KIF5A variant protein; Kinesin heavy chain isoform 5A; Kinesin heavy chain neuron-specific 1; kinesin, heavy chain, neuron-specific; Neuronal kinesin heavy chain; NKHC
Gene Aliases: BOS_5453; D10Bwg0738e; D12S1889; Khc; Kiaa4086; Kif5; KIF5A; Kns; mKIAA4086; MY050; NKHC; NKHC1; SPG10
UniProt ID: (Human) Q12840, (Mouse) P33175
Entrez Gene ID: (Human) 3798, (Bovine) 511273, (Mouse) 16572
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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