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Product Details
MA1-19352
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
KN-02
Immunogen
Enriched fraction of porcine brain kinesin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
TBS, pH 8
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2132396
Product Specific Information
This antibody recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.
Target Information
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Conventional kinesin heavy chain; epididymis secretory protein Li 61; kif5b {ECO:0000312|EMBL:ABC25059.1}; kinesin 1 (110-120kD); kinesin heavy chain; kinesin heavy chain member 5B, ubiquitous; Kinesin-1 heavy chain; U-KHC; Ubiquitous kinesin heavy chain; UKHC
Gene Aliases: AL022807; HEL-S-61; Khc; Khcs; KIF5B; KINH; KNS; KNS1; UKHC
UniProt ID: (Human) P33176, (Mouse) Q61768, (Rat) Q2PQA9
Entrez Gene ID: (Pig) 595132, (Human) 3799, (Mouse) 16573, (Rat) 117550
microtubule-based movement
cytoplasm organization
axon guidance
cytoskeleton-dependent intracellular transport
positive regulation of synaptic transmission, GABAergic
stress granule disassembly
regulation of membrane potential
positive regulation of potassium ion transport
vesicle transport along microtubule
plus-end-directed vesicle transport along microtubule
positive regulation of establishment of protein localization to plasma membrane
cell-cell adhesion
mitochondrial transport
microtubule-based process
protein localization
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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