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Invitrogen
Kir3.4 (KCNJ5) Polyclonal Antibody
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FIGURE: 1 / 9
Kir3.4 (KCNJ5) Antibody (PA5-119662) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Kir3.4 in HepG2 cells (green) using Kir3.4 (KCNJ5) Polyclonal Antibody (Product # PA5-119662). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
PA5-119662
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within N-terminal human Kir3.4
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913235
Product Specific Information
Positive Control: Mouse heart tissue, rat heart tissue, 293T, HepG2, LOVO, human lung cancer tissue, human pancreas tissue, mouse pancreas tissue.
Subcellular Location: Membrane.
Target Information
G protein-coupled inwardly rectifying potassium channels (Kir3.1 through Kir3.4) are coupled to numerous neurotransmitter receptors in the brain and are abundantly expressed in the olfactory bulb, hippocampus, neocortex, dentate gyrus, cerebellar cortex and thalamus regions of the brain. Also known as GIRK, Kir3 potassium channels localize to the soma and dendrites as well as axons of neurons. Liberated Gbg subunits from G protein heterotrimers bind to and regulate Kir3 channel activity. Gb3- and Gb4-containing Gbg dimers bind directly to cytoplasmic domains of Kir3 proteins and increase the K+ current while Gb5-containing Gbg dimers inhibit Kir3 K+ current. Kir3 activity is also inhibited by tyrosine phosphorylation. Brain-derived neurotrophic factor activates receptor tyrosine kinase B, which then phosphorylates Kir3 tyrosine residues, effectively inactivating the Kir3 channels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cardiac ATP-sensitive potassium channel; Cardiac inward rectifier; CIR; G protein-activated inward rectifier potassium channel 4; GIRK-4; GIRK4; Heart KATP channel; Inward rectifier K(+) channel Kir3.4; inward rectifier K+ channel KIR3.4; IRK-4; IRK4; KATP-1; Potassium channel, inwardly rectifying subfamily J member 5; potassium channel, inwardly rectifying subfamily J, member 5; potassium inwardly-rectifying channel, subfamily J, member 5
Gene Aliases: CIR; GIRK4; KATP1; KCNJ5; KIR3.4; LQT13
UniProt ID: (Human) P48544, (Mouse) P48545, (Rat) P48548
Entrez Gene ID: (Human) 3762, (Mouse) 16521, (Rat) 29713
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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