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Klotho Antibody (500-P296BT-1MG) in WB
Western Blot: To detect hKlotho by Western Blot analysis Klotho Polyclonal Antibody, Biotin (Product # 500-P296BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hKlotho is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P296BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Klotho
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930281
Product Specific Information
AA Sequence of recombinant protein: EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Klotho. Anti-Human Klotho-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hKlotho by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Klotho (500-P296) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hKlotho.
Western Blot: To detect hKlotho by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hKlotho is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P296BT-1MG will be provided as 2 x 500 µg
Target Information
KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HFTC3; Klotho
Gene Aliases: KL
UniProt ID: (Human) Q9UEF7
Entrez Gene ID: (Human) 9365
beta-glucuronidase activity
signal transducer activity
Ras guanyl-nucleotide exchange factor activity
fibroblast growth factor receptor binding
hormone activity
vitamin D binding
beta-glucosidase activity
1-phosphatidylinositol-3-kinase activity
fibroblast growth factor binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
MAPK cascade
carbohydrate metabolic process
energy reserve metabolic process
aging
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of bone mineralization
phosphatidylinositol-3-phosphate biosynthetic process
positive regulation of GTPase activity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
calcium ion homeostasis
positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway
glycosyl compound metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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