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L-Thyroxine T4 Antibody (MA5-14716) in WB
Western blot analysis of L-Thyroxine was performed by loading 600 ng, 300 ng, 150 ng and 75 ng of a Thyroxine-BSA conjugate and 10 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% milk in TBST for at least 1 hour at room temperature. L- Thyroxine was detected at ~62 kD using a L-Thyroxine monoclonal antibody antibody (Product # MA5-14716 (T4YCH clone8)) at... View More
Product Details
MA5-14716
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
T4YCH
Immunogen
Thyroxine-BSA
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10984550
Product Specific Information
MA5-14716 targets L-Thyroxine (T4) in ELISA, Western Blot, IP, and RIA applications and shows reactivity with Chemical samples.
Product MA514716 is a smaller package size of MIT0501 (formerly sold as a Seradyn product).
Target Information
Levothyroxine, also L-thyroxine, synthetic T4, or 3,5,3',5'-tetraiodo-L-thyronine, is a synthetic form of thyroxine (thyroid hormone), used as a hormone replacement for patients with thyroid problems. The natural hormone is chemically in the chiral L-form, as is the pharmaceutical agent. Dextrothyroxine (D-thyroxine) briefly saw research as an anticholesterol agent but was pulled due to cardiac side-effects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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