Proteintech

L2HGDH Polyclonal Antibody

L2HGDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

L2HGDH Antibody (15707-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 15707-1-AP) (L2HGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

15707-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

L2HGDH Fusion Protein Ag8382 (1-351 aa encoded by BC006117)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MVPALRYLV GACGRARGRF AGGSPGACGF ASGRPRPLCG GSRSASTSSF DIVIVGGGIV GLASARALIL RHPSLSIGVL EKEKDLAVHQ TGHNSGVIHS GIYYKPESLK AKLCVQGAAL LYEYCQQKGI SYKQCGKLIV AVEQEEIPRL QALYEKGLQN GVPGLRLIQQ EDIKKKEPYC RGLMAIDCPH TGIVDYRQVA LSFAQDFQEA GGSVLTNFEV KGIEMAKESP SRSIDGMQYP IVIKNTKGEE IRCQYVVTCA GLYSDRISEL SGCTPDPRIV PFRGDYLLLK PEKCYLVKGN IYPVPDSRFP FLGVHFTPRM DGSIWLGPNA VLAFKREGYR PFDFSATDVM DI (1-351 aa encoded by BC006117)

Target Information

This gene encodes L-2-hydroxyglutarate dehydrogenase, a FAD-dependent enzyme that oxidizes L-2-hydroxyglutarate to alpha-ketoglutarate in a variety of mammalian tissues. Mutations in this gene cause L-2-hydroxyglutaric aciduria, a rare autosomal recessive neurometabolic disorder resulting in moderate to severe mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

L-2hydroxyglutaric acid rewires amino acid metabolism in colorectal cancer via the mTOR-ATF4 axis.

Oncogene

Tabata S,Kojima Y,Sakamoto T,Igarashi K,Umetsu K,Ishikawa T,Hirayama A,Kajino-Sakamoto R,Sakamoto N,Yasumoto KI,Okano K,Suzuki Y,Yachida S,Aoki M,Soga T

Sat Apr 01 00:00:00 UTC 2023

Oncometabolite d-2HG alters T cell metabolism to impair CD8⁺ T cell function.

Science (New York, N.Y.)

Notarangelo G,Spinelli JB,Perez EM,Baker GJ,Kurmi K,Elia I,Stopka SA,Baquer G,Lin JR,Golby AJ,Joshi S,Baron HF,Drijvers JM,Georgiev P,Ringel AE,Zaganjor E,McBrayer SK,Sorger PK,Sharpe AH,Wucherpfennig KW,Santagata S,Agar NYR,Suvà ML,Haigis MC

Fri Sep 30 00:00:00 UTC 2022

MYC, mitochondrial metabolism and O-GlcNAcylation converge to modulate the activity and subcellular localization of DNA and RNA demethylases.

Leukemia

Lin AP,Qiu Z,Ethiraj P,Sasi B,Jaafar C,Rakheja D,Aguiar RCT

Fri Apr 01 00:00:00 UTC 2022

Renal oncometabolite L-2-hydroxyglutarate imposes a block in kidney tubulogenesis: Evidence for an epigenetic basis for the L-2HG-induced impairment of differentiation.

Frontiers in endocrinology

Taub M,Mahmoudzadeh NH,Tennessen JM,Sudarshan S

Fri Sep 23 00:00:00 UTC 2022

Urinary 2-Hydroxyglutarate Enantiomers Are Markedly Elevated in a Murine Model of Type 2 Diabetic Kidney Disease.

Metabolites

Baek J,Pennathur S

Wed Jul 21 00:00:00 UTC 2021

Metabolic remodelling during early mouse embryo development.

Nature metabolism

Zhao J,Yao K,Yu H,Zhang L,Xu Y,Chen L,Sun Z,Zhu Y,Zhang C,Qian Y,Ji S,Pan H,Zhang M,Chen J,Correia C,Weiskittel T,Lin DW,Zhao Y,Chandrasekaran S,Fu X,Zhang D,Fan HY,Xie W,Li H,Hu Z,Zhang J

Fri Oct 01 00:00:00 UTC 2021

MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome.

Cell chemical biology

Qiu Z,Lin AP,Jiang S,Elkashef SM,Myers J,Srikantan S,Sasi B,Cao JZ,Godley LA,Rakheja D,Lyu Y,Zheng S,Madesh M,Shiio Y,Dahia PLM,Aguiar RCT

Thu May 21 00:00:00 UTC 2020

Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Shelar S,Shim EH,Brinkley GJ,Kundu A,Carobbio F,Poston T,Tan J,Parekh V,Benson D,Crossman DK,Buckhaults PJ,Rakheja D,Kirkman R,Sato Y,Ogawa S,Dutta S,Velu SE,Emberley E,Pan A,Chen J,Huang T,Absher D,Becker A,Kunick C,Sudarshan S

Sat Dec 15 00:00:00 UTC 2018

2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity.

Science translational medicine

Sulkowski PL,Corso CD,Robinson ND,Scanlon SE,Purshouse KR,Bai H,Liu Y,Sundaram RK,Hegan DC,Fons NR,Breuer GA,Song Y,Mishra-Gorur K,De Feyter HM,de Graaf RA,Surovtseva YV,Kachman M,Halene S,Günel M,Glazer PM,Bindra RS

Wed Feb 01 00:00:00 UTC 2017

2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity.

Science translational medicine

Wed Feb 01 00:00:00 UTC 2017

L2hgdh Deficiency Accumulates l-2-Hydroxyglutarate with Progressive Leukoencephalopathy and Neurodegeneration.

Molecular and cellular biology

Ma S,Sun R,Jiang B,Gao J,Deng W,Liu P,He R,Cui J,Ji M,Yi W,Yang P,Wu X,Xiong Y,Qiu Z,Ye D,Guan KL

Sat Apr 15 00:00:00 UTC 2017

L2hgdh Deficiency Accumulates l-2-Hydroxyglutarate with Progressive Leukoencephalopathy and Neurodegeneration.

Molecular and cellular biology

Ma S,Sun R,Jiang B,Gao J,Deng W,Liu P,He R,Cui J,Ji M,Yi W,Yang P,Wu X,Xiong Y,Qiu Z,Ye D,Guan KL

Sat Apr 15 00:00:00 UTC 2017

Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.

Cell metabolism

Oldham WM,Clish CB,Yang Y,Loscalzo J

Tue Aug 04 00:00:00 UTC 2015

Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.

Cell metabolism

Oldham WM,Clish CB,Yang Y,Loscalzo J

Tue Aug 04 00:00:00 UTC 2015

Hypoxia Induces Production of L-2-Hydroxyglutarate.

Cell metabolism

Intlekofer AM,Dematteo RG,Venneti S,Finley LW,Lu C,Judkins AR,Rustenburg AS,Grinaway PB,Chodera JD,Cross JR,Thompson CB

Tue Aug 04 00:00:00 UTC 2015

Hypoxia Induces Production of L-2-Hydroxyglutarate.

Cell metabolism

Intlekofer AM,Dematteo RG,Venneti S,Finley LW,Lu C,Judkins AR,Rustenburg AS,Grinaway PB,Chodera JD,Cross JR,Thompson CB

Tue Aug 04 00:00:00 UTC 2015

D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth.

Oncotarget

Ma S,Jiang B,Deng W,Gu ZK,Wu FZ,Li T,Xia Y,Yang H,Ye D,Xiong Y,Guan KL

Mon Apr 20 00:00:00 UTC 2015

L-2-Hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer.

Cancer discovery

Shim EH,Livi CB,Rakheja D,Tan J,Benson D,Parekh V,Kho EY,Ghosh AP,Kirkman R,Velu S,Dutta S,Chenna B,Rea SL,Mishur RJ,Li Q,Johnson-Pais TL,Guo L,Bae S,Wei S,Block K,Sudarshan S

Sat Nov 01 00:00:00 UTC 2014

L-2-Hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer.

Cancer discovery

Shim EH,Livi CB,Rakheja D,Tan J,Benson D,Parekh V,Kho EY,Ghosh AP,Kirkman R,Velu S,Dutta S,Chenna B,Rea SL,Mishur RJ,Li Q,Johnson-Pais TL,Guo L,Bae S,Wei S,Block K,Sudarshan S

Sat Nov 01 00:00:00 UTC 2014

Screen for IDH1, IDH2, IDH3, D2HGDH and L2HGDH mutations in glioblastoma.

PloS one

Krell D,Assoku M,Galloway M,Mulholland P,Tomlinson I,Bardella C

Mon Nov 28 00:00:00 UTC 2011

Screen for IDH1, IDH2, IDH3, D2HGDH and L2HGDH mutations in glioblastoma.

PloS one

Krell D,Assoku M,Galloway M,Mulholland P,Tomlinson I,Bardella C

Mon Nov 28 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: 2-hydroxyglutarate dehydrogenase; alpha-hydroxyglutarate oxidoreductase; alpha-ketoglutarate reductase; Duranin; FLJ12618; L-2-hydroxyglutarate dehydrogenase, mitochondrial; L-alpha-hydroxyglutarate dehydrogenase

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Gene Aliases: BC016226; C14orf160; L2HGA; L2HGDH; RGD1306250

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UniProt ID: (Human) Q9H9P8, (Mouse) Q91YP0

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Entrez Gene ID: (Human) 79944, (Mouse) 217666, (Rat) 314196

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Function(s)

2-hydroxyglutarate dehydrogenase activity oxidoreductase activity

Process(es)

2-oxoglutarate metabolic process cellular protein metabolic process oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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L2HGDH Polyclonal Antibody

L2HGDH Antibody (15707-1-AP) in IHC (P)

Product Details

15707-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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