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OriGene
LAMA4 Monoclonal Antibody (OTI4A8), TrueMAB™
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FIGURE: 1 / 1
LAMA4 Antibody (CF805722) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAMA4 (RC226434, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-LAMA4. Positive lysates LY426227 (100 µg) and LC426227 (20 µg) can be purchased separately from OriGene.
Product Details
CF805722
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI4A8
Immunogen
Human recombinant protein fragment corresponding to amino acids 25-270 of human LAMA4 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Laminin is a non-collagenous protein of basement membranes. By rotary shadowing it has a cross-like shape, consisting of one long arm, (400 kD) and three short arms (200 kD). Antibodies to laminin localize exclusively in basement membranes. Laminin is found throughout the basement membrane, but the reaction with the antibodies to laminin is more intense in the lamina lucida, the part of the basement membrane adjacent to the cell membrane. Laminin binds to type IV collagen, a component exclusively localized in the basement membrane. Laminin has been shown to play a role in cell adhesion and attachment in vivo and in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: laminin alpha 4 chain; Laminin subunit alpha-4; laminin, alpha 4; Laminin-14 subunit alpha; Laminin-8 subunit alpha; Laminin-9 subunit alpha
Gene Aliases: CMD1JJ; LAMA3; LAMA4; LAMA4*-1
UniProt ID: (Human) Q16363
Entrez Gene ID: (Human) 3910
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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