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LAMP1 Monoclonal Antibody (H4A3), Alexa Fluor™ 488

LAMP1 Antibody in Immunocytochemistry (ICC/IF)

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LAMP1 Antibody (MA5-18121) in ICC/IF

Knockdown of LAMP1 was achieved by transfecting HeLa cells with LAMP1 specific siRNAs (Silencer® select Product # s8080, s8081 ). Immunofluorescence analysis was performed using untransfected HeLa cells (panels a, d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with LAMP1 specific siRNAs (panel c,f). Cells were fixed, permeabilized, and probed with LAMP1 Monoclonal Antibody (H4A3), Alexa Fluor 488 (Product # MA5-18121, 1:250 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with D... View More

Product Image Gallery

LAMP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with LAMP-1 siRNA and reduction of signal was observed in Immunofluorescence using LAMP1 Monoclonal Antibody (H4A3), Alexa Fluor 488 (Product # MA5-18121). {KD}

Product Details

MA5-18121

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

4 µL/100 µL whole blood or 10^6 cells

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

H4A3

Immunogen

Human PBMC

View immunogen

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2539495

Product Specific Information

This antibody recognizes an extracellular/luminal epitope of CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.

Target Information

LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer’s Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

An Optimally Fabricated Platform Guides Cancer-Specific Activation of Chemotherapeutic Drugs and Toxicity-Free Cancer Treatment.

Advanced healthcare materials

Moon OJ,Yoon CJ,Lee BR,Lee J

MA5-18121 was used in Immunocytochemistry-immunofluorescence to find that cancer chemotherapeutic drugs dramatically reduces drug toxicity to normal cells, significantly enhances efficacy in in vivo cancer treatment without toxicity to normal organs of mice, and thus is expected to open up a novel clinical route to break through the limits of current cancer chemotherapy.

Mon Aug 01 00:00:00 UTC 2022

Elevated glucosylsphingosine in Gaucher disease induced pluripotent stem cell neurons deregulates lysosomal compartment through mammalian target of rapamycin complex 1.

Stem cells translational medicine

Srikanth MP,Jones JW,Kane M,Awad O,Park TS,Zambidis ET,Feldman RA

MA5-18121 was used in Western Blot, Immunocytochemistry to identify the role of lipid in Gaucher disease using human induced pluripotent stem cell-derived neurons from types 2 and 3 neuronopathic GD (nGD) patients.

Thu Jul 01 00:00:00 UTC 2021

Elevated glucosylsphingosine in Gaucher disease induced pluripotent stem cell neurons deregulates lysosomal compartment through mammalian target of rapamycin complex 1.

Stem cells translational medicine

Srikanth MP,Jones JW,Kane M,Awad O,Park TS,Zambidis ET,Feldman RA

Thu Jul 01 00:00:00 UTC 2021

Quantification of natural killer cell polarization and visualization of synaptic granule externalization by imaging flow cytometry.

Clinical immunology (Orlando, Fla.)

Viswanath DI,Mace EM,Hsu HT,Orange JS

MA5-18121 was used in Flow cytometry/Cell sorting to develop and describe the use of imaging flow cytometry (IFC) in the high throughput, rapid analysis of natural killer cell function.

Sat Apr 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: 120 kDa lysosomal membrane glycoprotein; CD107 antigen-like family member A; CD107a; Lamp I; LAMP-1; LGP-120; LGP-A; lysosomal membrane glycoprotein 1; Lysosomal membrane glycoprotein A; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); lysosome-associated membrane protein 1; P2B

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Gene Aliases: AI196048; CD107a; Lamp-1; LAMP1; LAMPA; LGP120

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UniProt ID: (Human) P11279, (Mouse) P11438

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Entrez Gene ID: (Human) 3916, (Mouse) 16783

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Function(s)

virus receptor activity protein binding enzyme binding protein domain specific binding

Process(es)

spermatogenesis granzyme-mediated apoptotic signaling pathway positive regulation of natural killer cell degranulation positive regulation of natural killer cell mediated cytotoxicity autophagic cell death protein stabilization establishment of protein localization to organelle Golgi to lysosome transport regulation of organelle transport along microtubule autophagy viral entry into host cell

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LAMP1 Monoclonal Antibody (H4A3), Alexa Fluor™ 488

LAMP1 Antibody (MA5-18121) in ICC/IF

Product Details

MA5-18121

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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