Invitrogen

LAMP1 Monoclonal Antibody (LY1C6)

LAMP1 Antibody in Immunocytochemistry (ICC/IF)

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LAMP1 Antibody (MA1-164) in ICC/IF

Immunofluorescence analysis of LAMP1 (Lysosome-associated membrane glycoprotein 1) was performed using 80% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LAMP1 Monoclonal Antibody (LY1C6) (Product # MA1-164) at 10 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Ant... View More

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LAMP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. L6 cells were transfected with LAMP1 siRNA and decrease in signal intensity was observed in ICC application using Anti-LAMP1 Monoclonal Antibody (LY1C6) (Product # MA1-164). {KD}

Product Details

MA1-164

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

View 4 publications 4 publications

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

LY1C6

Immunogen

Rat liver lysosomal membrane preparations

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536869

Product Specific Information

MA1-164 detects glycosylated LAMP-1 at ~130kD.

M-PER (Product # 78501), IP Lysis Buffer (Product # 87787), or a 1% Triton X-100 lysis buffer are recommended for Western blot. LAMP-1 was not detected when RIPA buffer was used to generate cell lysates.

Target Information

LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer’s Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ADSCs increase the autophagy of chondrocytes through decreasing miR-7-5p in Osteoarthritis rats by targeting ATG4A.

International immunopharmacology

Zhao S,Liu Y,Wang J,Wen Y,Wu B,Yang D,Wang G,Xiu G,Ling B,Du D,Xu J

MA1-164 was used in Western Blot to demonstrate a potential role for ADSCs based therapeutics for preventing of articular cartilage destruction and extracellular matrix (ECM) degradation in OA.

Sat Jul 01 00:00:00 UTC 2023

Vibrio cholerae Porin OmpU Activates Dendritic Cells via TLR2 and the NLRP3 Inflammasome.

Infection and immunity

Dhar V,Gandhi S,Sakharwade SC,Chawla A,Mukhopadhaya A

MA1-164 was used in Western Blot to reveal that OmpU-mediated activation of TLR2 induces signaling via PKC, MAPKs p38 and extracellular signal-regulated kinase (ERK), and transcription factor NF-κB; however, PI3K and MAPK Jun N-terminal protein kinase (JNK) are activated in TLR2 independent manner.

Thu Feb 16 00:00:00 UTC 2023

VAMP4 regulates insulin levels by targeting secretory granules to lysosomes.

The Journal of cell biology

Li M,Feng F,Feng H,Hu P,Xue Y,Xu T,Song E

MA1-164 was used in Western Blot to identify that VAMP4 controlled the insulin levels in response to glucose challenge.

Mon Oct 03 00:00:00 UTC 2022

Prolonged ethanol exposure modulates constitutive internalization and recycling of 5-HT1A receptors.

Journal of neurochemistry

Wang S,Liu H,Roberts JB,Wiley AP,Marayati BF,Adams KL,Luessen DJ,Eldeeb K,Sun H,Zhang K,Chen R

MA1-164 was used in Immunocytochemistry-immunofluorescence to suggest that prolonged ethanol exposure not only alters the endocytic trafficking of 5-HT1ARs but also the mechanism by which constitutive 5-HT1AR internalization occurs.

Tue Feb 01 00:00:00 UTC 2022

DCN released from ferroptotic cells ignites AGER-dependent immune responses.

Autophagy

Liu J,Zhu S,Zeng L,Li J,Klionsky DJ,Kroemer G,Jiang J,Tang D,Kang R

MA1-164 was used in Western Blotting to report that the proteoglycan decorin (DCN) is released by cells that are dying from ferroptosis and then acts as an alarm signal to trigger innate and adaptive immune responses.

Thu Sep 01 00:00:00 UTC 2022

DCN released from ferroptotic cells ignites AGER-dependent immune responses.

Autophagy

Liu J,Zhu S,Zeng L,Li J,Klionsky DJ,Kroemer G,Jiang J,Tang D,Kang R

Thu Sep 01 00:00:00 UTC 2022

KIF5A-dependent axonal transport deficiency disrupts autophagic flux in trimethyltin chloride-induced neurotoxicity.

Autophagy

Liu M,Pi H,Xi Y,Wang L,Tian L,Chen M,Xie J,Deng P,Zhang T,Zhou C,Liang Y,Zhang L,He M,Lu Y,Chen C,Yu Z,Zhou Z

MA1-164 was used in Western Blot to investigate the mechanism of induction of neurotoxicity by trimethyltin chloride.

Thu Apr 01 00:00:00 UTC 2021

Endolysosomal ion channel MCOLN2 (Mucolipin-2) promotes prostate cancer progression via IL-1β/NF-κB pathway.

British journal of cancer

Yu H,Xie M,Meng Z,Lo CY,Chan FL,Jiang L,Meng X,Yao X

MA1-164 was used in Immunocytochemistry to highlight an intriguing possibility of targeting MCOLN2 as potential therapeutic strategy in Pca treatment.

Mon Nov 01 00:00:00 UTC 2021

Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.

Nature communications

Dankovich TM,Kaushik R,Olsthoorn LHM,Petersen GC,Giro PE,Kluever V,Agüi-Gonzalez P,Grewe K,Bao G,Beuermann S,Hadi HA,Doeren J,Klöppner S,Cooper BH,Dityatev A,Rizzoli SO

MA1-164 was used in Immunocytochemistry, Western Blot, Immunoprecipitation to conclude that the neuronal ECM can be remodeled frequently through mechanisms that involve endocytosis and recycling of ECM proteins.

Wed Dec 08 00:00:00 UTC 2021

Extracellular and Luminal pH Regulation by Vacuolar H+-ATPase Isoform Expression and Targeting to the Plasma Membrane and Endosomes.

The Journal of biological chemistry

Smith GA,Howell GJ,Phillips C,Muench SP,Ponnambalam S,Harrison MA

MA1-164 was used in western blot to investigate luminal and extracellular pH regulation by Vacuolar H+-ATPase isoform expression and targeting to the endosomes and plasma membrane

Fri Apr 15 00:00:00 UTC 2016

Extracellular and Luminal pH Regulation by Vacuolar H+-ATPase Isoform Expression and Targeting to the Plasma Membrane and Endosomes.

The Journal of biological chemistry

Smith GA,Howell GJ,Phillips C,Muench SP,Ponnambalam S,Harrison MA

MA1-164 was used in western blot to investigate luminal and extracellular pH regulation by Vacuolar H+-ATPase isoform expression and targeting to the endosomes and plasma membrane

Fri Apr 15 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: 120 kDa lysosomal membrane glycoprotein; CD107 antigen-like family member A; CD107a; Lamp I; LAMP-1; LGP-120; LGP-A; Lysosomal associated membrane protein 1 (120 kDa); lysosomal membrane glycoprotein 1; Lysosomal membrane glycoprotein A; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); Lysosome-associated membrane protein 1; P2B

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Gene Aliases: AI196048; CD107a; I79_011073; LAMP-1; LAMP1; LAMPA; LGP-A; LGP120; LGPA

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UniProt ID: (Human) P11279, (Chinese hamster) P49129, (Mouse) P11438, (Rat) P14562

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Entrez Gene ID: (Human) 3916, (Chinese hamster) 100689406, (Mouse) 16783, (Rat) 25328

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Function(s)

virus receptor activity protein binding enzyme binding protein domain specific binding molecular_function

Process(es)

spermatogenesis granzyme-mediated apoptotic signaling pathway positive regulation of natural killer cell degranulation positive regulation of natural killer cell mediated cytotoxicity autophagic cell death protein stabilization establishment of protein localization to organelle Golgi to lysosome transport regulation of organelle transport along microtubule autophagy viral entry into host cell

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LAMP1 Monoclonal Antibody (LY1C6)

LAMP1 Antibody (MA1-164) in ICC/IF

Product Details

MA1-164

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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