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LAR4B Antibody (PA5-143989) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of LAR4B in A431 cells using LAR4B Polyclonal Antibody (Product # PA5-143989). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter se... View More
Product Details
PA5-143989
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human LARP4B recombinant protein (Position: R241-K684).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075203
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HEK293 whole cell, human Jurkat whole cell, human K562 whole cell. IHC: human appendiceal adenocarcinoma tissue, human liver cancer tissue. ICC/IF: A431 cell. Flow: THP-1 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The LAR4B gene, more accurately referred to as LARP4B (La Ribonucleoprotein Domain Family Member 4B), is associated with RNA-binding proteins that are crucial for various aspects of RNA metabolism, including RNA stabilization, translation, and cellular stress responses. LARP4B belongs to the La-related protein family, which plays significant roles in maintaining RNA stability and enhancing translation efficiency of certain mRNAs. It has been identified as a target in cancer research due to its involvement in tumor biology, particularly regulating tumor suppressive pathways and interacting with microRNA networks. Studies have shown that LARP4B expression is downregulated in prostate cancer tissues, indicating its potential role as a tumor suppressor. Moreover, it is linked with liver cancer and survival rates, reflecting its importance in cellular growth and proliferation. The regulation of LARP4B and its interactions with other cellular components underline its potential therapeutic implications for cancer treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: La ribonucleoprotein domain family member 4B; La ribonucleoprotein domain family member 5; La ribonucleoprotein domain family, member 4B; La ribonucleoprotein domain family, member 5; La-related protein 4B; La-related protein 5
Gene Aliases: KIAA0217; LARP4B; LARP5
UniProt ID: (Human) Q92615
Entrez Gene ID: (Human) 23185
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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