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OriGene
LATS2 Monoclonal Antibody (OTI2D3), TrueMAB™
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FIGURE: 1 / 1
LATS2 Antibody (CF804435) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LATS2 (RC219394, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-LATS2. Positive lysates LY402348 (100 µg) and LC402348 (20 µg) can be purchased separately from OriGene.
Product Details
CF804435
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2D3
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-237 of human LATS2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
LATS2 is a serine/threonine protein kinase belonging to the LATS tumor suppressor family. LATS2 interacts with a negative regulator of p53 and function in a positive feedback loop with p53 that responds to cytoskeleton damage and this interaction provokes centrosome/mitotic apparatus dysfunction. LATS2 plays an essential role in the maintenance of mitotic fidelity and genomic integrity. LATS2 plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. It negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: h-warts; Kinase phosphorylated during mitosis protein; Large tumor suppressor homolog 2; LATS (large tumor suppressor, Drosophila) homolog 2; LATS, large tumor suppressor, homolog 2; LATS2; serine/threonine kinase KPM; Serine/threonine-protein kinase kpm; Serine/threonine-protein kinase LATS2; Warts-like kinase
Gene Aliases: KPM; LATS2
UniProt ID: (Human) Q9NRM7
Entrez Gene ID: (Human) 26524
G1/S transition of mitotic cell cycle
inner cell mass cell fate commitment
inner cell mass cellular morphogenesis
protein phosphorylation
mitotic nuclear division
hormone-mediated signaling pathway
peptidyl-serine phosphorylation
keratinocyte differentiation
cellular protein localization
hippo signaling
intracellular signal transduction
positive regulation of apoptotic process
negative regulation of cyclin-dependent protein serine/threonine kinase activity
regulation of organ growth
cell division
negative regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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