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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • LC3A/LC3B Antibodies

          Proteintech

          LC3 Polyclonal Antibody

          1,040 References
          View all (13) LC3A/LC3B antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite LC3 Polyclonal Antibody

          • Antibody Testing Data (29)
          LC3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          LC3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 29

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          LC3 Antibody (14600-1-AP) in ICC/IF

          Immunofluorescent analysis of Starvation treated HEK-293 cells using 14600-1-AP (LC3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). 20 mM chloroquine was used to block the autophagy flux. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          LC3 Antibody in Immunocytochemistry (ICC/IF)
          LC3 Antibody in Immunocytochemistry (ICC/IF)
          LC3 Antibody in Immunocytochemistry (ICC/IF)
          LC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Western Blot (WB)
          LC3 Antibody in Flow Cytometry (Flow)
          LC3 Antibody in Immunoprecipitation (IP)

          Product Details

          14600-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 744 publications 744 publications

          Immunohistochemistry (IHC)

          -
          View 82 publications 82 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 206 publications 206 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 3 publications 3 publications

          Not applicable (N/A)

          -
          View 5 publications 5 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Goat, Human, Mouse, Pig, Rat, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          LC3 Fusion Protein Ag6144 (1-125 aa encoded by BC067797)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.27 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

          Immunogen sequence: MPSEKTFKQ RRTFEQRVED VRLIREQHPT KIPVIIERYK GEKQLPVLDK TKFLVPDHVN MGELIKIIRR RLQLNANQAF FLLVNGHSMV SVSTPISEVY ESEKDEDGFL YMVYASQETF GMKLSV (1-125 aa encoded by B C067797)

          Target Information

          Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Autophagy-related protein LC3 A; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 A; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 1; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B LC3 B-like; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated protein 1 light chain 3 beta; Microtubule-associated protein 1 light chain 3 beta 2; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3 beta 2; Microtubule-associated proteins 1A/1B light chain 3A; Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like; Microtubule-associated proteins 1A/1B light chain 3B-like protein

          View more View less

          Gene Aliases: 1010001C15Rik; 1010001H21Rik; 4922501H04Rik; Atg8; ATG8E; ATG8F; ATG8G; LC3; LC3A; LC3B; MAP1A/1BLC3; MAP1ALC3; MAP1BLC3; Map1lc3; MAP1LC3A; MAP1LC3B; MAP1LC3B-a; MAP1LC3B2; Mpl3; zbs559

          View more View less

          UniProt ID: (Human) Q9H492, (Human) Q9GZQ8, (Human) A6NCE7, (Rat) Q6XVN8, (Mouse) Q91VR7, (Rat) Q62625, (Mouse) Q9CQV6

          View more View less

          Entrez Gene ID: (Human) 84557, (Human) 81631, (Human) 643246, (Rat) 362245, (Mouse) 66734, (Rat) 64862, (Mouse) 67443

          View more View less

          Function(s)
          protein binding phospholipid binding microtubule binding phosphatidylethanolamine binding ubiquitin protein ligase binding molecular_function GABA receptor binding tubulin binding protein domain specific binding
          Process(es)
          autophagosome assembly mitophagy cellular response to nitrogen starvation macroautophagy regulation of mitophagy autophagy positive regulation of protein binding nucleophagy membrane fusion positive regulation of mucus secretion
          It has to be done as per old AB suggested Products section.
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