Invitrogen

LC3B Recombinant Rabbit Monoclonal Antibody (2H30L32)

LC3B Antibody in Immunocytochemistry (ICC/IF)

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LC3B Antibody (700712) in ICC/IF

Immunofluorescent analysis of LC3B was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with LC3B Recombinant Rabbit Monoclonal Antibody (Product # 700712) at 2 µg/mL and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at r... View More

LC3B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

LC3B Antibody — Figure 5 Immunofluorescence images of LC3B-II in HCT116 and SW480 cells. Cells were treated with cb FeD-containing serum solutions prepared from mice treated by gastrogavage with saline (control), or 5 (low), 10 (mid) or 20 (high) g/kg of cb FeD, followed by immunofluorescence staining of LC3B-II (magnification, x200). Red, LC3B-II; blue, DAPI; Merge, LC3B-II+DAPI. cb FeD, Codonopis bulleynana Forest ex Diels; LC3, LC3, microtubule-associated protein 1 light chain 3. {TM}

Product Details

700712

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1-3 µg/mL

View 4 publications 4 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

1 µL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

2H30L32

Immunogen

Recombinant human LC3B protein corresponding to amino acids 2-120

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532340

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and important is in neurogenesis. Recent studies indicate that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component. Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). LC3B is a microtubule-associated protein that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3B may play a role in processes involving cancer, aging, metabolic and neurodegenerative disorders and cardiovascular/pulmonary diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Degradation of lipid droplets by chimeric autophagy-tethering compounds.

Cell research

Fu Y,Chen N,Wang Z,Luo S,Ding Y,Lu B

700712 was used in Western Blot to demonstrate the capability of degrading LDs by ATTECs.

Wed Sep 01 00:00:00 UTC 2021

Acid ceramidase controls apoptosis and increases autophagy in human melanoma cells treated with doxorubicin.

Scientific reports

Lai M,Amato R,La Rocca V,Bilgin M,Freer G,Spezia P,Quaranta P,Piomelli D,Pistello M

700712 was used in Western Blot to elucidate the role of acid ceramidase in chemotherapy resistance in melanoma cells.

Thu May 27 00:00:00 UTC 2021

Acid ceramidase controls apoptosis and increases autophagy in human melanoma cells treated with doxorubicin.

Scientific reports

Lai M,Amato R,La Rocca V,Bilgin M,Freer G,Spezia P,Quaranta P,Piomelli D,Pistello M

700712 was used in Western Blot to elucidate the role of acid ceramidase in chemotherapy resistance in melanoma cells.

Thu May 27 00:00:00 UTC 2021

Role of prostaglandin E2 receptor 4 in the modulation of apoptosis and mitophagy during ischemia/reperfusion injury in the kidney.

Molecular medicine reports

Ding C,Han F,Xiang H,Wang Y,Dou M,Xia X,Li Y,Zheng J,Ding X,Xue W,Tian P

700712 was used in Immunocytochemistry-immunoflourescence to investigate the protective effects of EP4 signaling on renal mitochondria and against renal IRI.

Tue Oct 01 00:00:00 UTC 2019

Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.

Nature

Li Z,Wang C,Wang Z,Zhu C,Li J,Sha T,Ma L,Gao C,Yang Y,Sun Y,Wang J,Sun X,Lu C,Difiglia M,Mei Y,Ding C,Luo S,Dang Y,Ding Y,Fei Y,Lu B

700712 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate the concept of lowering levels of disease-causing proteins using autophagosome-tethering compounds.

Fri Nov 01 00:00:00 UTC 2019

Codonopis bulleynana Forest ex Diels inhibits autophagy and induces apoptosis of colon cancer cells by activating the NF-κB signaling pathway.

International journal of molecular medicine

Luan Y,Li Y,Zhu L,Zheng S,Mao D,Chen Z,Cao Y

700712 was used in Immunocytochemistry-immunoflourescence to investigate the anti-cancer activity of odonopis bulleynana Forest ex Diels, and its potential molecular mechanisms.

Thu Mar 01 00:00:00 UTC 2018

Mannich Curcuminoids as Potent Anticancer Agents.

Archiv der Pharmazie

Gyuris M,Hackler L,Nagy LI,Alföldi R,Rédei E,Marton A,Vellai T,Faragó N,Ózsvári B,Hetényi A,Tóth GK,Sipos P,Kanizsai I,Puskás LG

Published figure using LC3B monoclonal antibody (Product # 700712) in Western Blot

Sat Jul 01 00:00:00 UTC 2017

A quantitative TR-FRET plate reader immunoassay for measuring autophagy.

Autophagy

Hancock MK,Hermanson SB,Dolman NJ

700712 was used in immunoprecipitation and western blot to develop a TR-FRET immuno-assay for the quantitative measurement of autophagy using GFP-tagged LC3B

Wed Aug 01 00:00:00 UTC 2012

A quantitative TR-FRET plate reader immunoassay for measuring autophagy.

Autophagy

Hancock MK,Hermanson SB,Dolman NJ

700712 was used in immunoprecipitation and western blot to develop a TR-FRET immuno-assay for the quantitative measurement of autophagy using GFP-tagged LC3B

Wed Aug 01 00:00:00 UTC 2012

A quantitative TR-FRET plate reader immunoassay for measuring autophagy.

Autophagy

Hancock MK,Hermanson SB,Dolman NJ

700712 was used in immunoprecipitation and western blot to develop a TR-FRET immuno-assay for the quantitative measurement of autophagy using GFP-tagged LC3B

Wed Aug 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: ATG8E; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3 beta; LC3A; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 B; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B LC3 B-like; MAP1A/MAP1B light chain 3 B; MAP1BLC3; MAP1LC3A; Microtubule-associated protein 1 light chain 3 beta; Microtubule-associated protein 1 light chain 3 beta 2; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3 beta 2; Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like; Microtubule-associated proteins 1A/1B light chain 3B-like protein; RP11-346K17.1

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Gene Aliases: 1010001C15Rik; Atg8; ATG8F; ATG8G; LC3B; MAP1A/1BLC3; MAP1ALC3; Map1lc3; MAP1LC3B; MAP1LC3B-a; MAP1LC3B2; Mpl3; zbs559

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UniProt ID: (Human) Q9GZQ8, (Human) A6NCE7, (Rat) Q62625, (Mouse) Q9CQV6

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Entrez Gene ID: (Human) 81631, (Human) 643246, (Rat) 64862, (Mouse) 67443

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Function(s)

protein binding microtubule binding ubiquitin protein ligase binding tubulin binding protein domain specific binding GABA receptor binding

Process(es)

autophagosome assembly cellular response to nitrogen starvation macroautophagy regulation of mitophagy mitophagy autophagy positive regulation of protein binding nucleophagy membrane fusion positive regulation of mucus secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LC3B Recombinant Rabbit Monoclonal Antibody (2H30L32)

LC3B Antibody (700712) in ICC/IF

Product Details

700712

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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