Rockland

LDB1 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-350

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1:425,000
-

Immunoprecipitation (IP)

1:500-1:3,000
-

ChIP assay (ChIP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of mouse LDB1 protein.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.8 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against the mouse LDB1protein. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, frog, chicken and rat based on 100% homology for the immunogen sequence. Cross reactivity with LDB1 homologues from other sources has not been determined.

Target Information

LIM domain-binding protein 1 (LDB1), also known as Carboxyl-terminal LIM domain-binding protein 2 (CLIM2), binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. LDB1 may regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. It plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. LDB1 acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. It also acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state. LDB1 strongly interacts with the LIM2 domain of LMX1A to form homodimer. LDB1 protein is expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Due to alternative splicing events, three isoforms exist for LDB1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: carboxy terminal LIM domain protein 2; Carboxyl-terminal LIM domain-binding protein 2; CLIM-2; hLdb1; LDB-1; LIM domain-binding factor CLIM2; LIM domain-binding factor-1; LIM domain-binding protein 1; mLdb1; Nuclear LIM interactor

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Gene Aliases: CLIM-2; CLIM2; LDB-1; LDB1; NLI

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UniProt ID: (Human) Q86U70, (Mouse) P70662

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Entrez Gene ID: (Human) 8861, (Rat) 309447, (Mouse) 16825

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Function(s)
RNA polymerase II activating transcription factor binding enhancer sequence-specific DNA binding chromatin binding transcription corepressor activity protein binding enzyme binding LIM domain binding protein homodimerization activity protein self-association transcription factor activity, transcription factor binding DNA binding transcription cofactor activity
Process(es)
transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery gastrulation with mouth forming second hair follicle development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development anterior/posterior axis specification epithelial structure maintenance Wnt signaling pathway cerebellar Purkinje cell differentiation cellular component assembly neuron differentiation regulation of cell migration regulation of DNA-templated transcription, elongation somatic stem cell population maintenance regulation of kinase activity histone H3-K4 acetylation negative regulation of erythrocyte differentiation positive regulation of cell adhesion negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hemoglobin biosynthetic process regulation of focal adhesion assembly cerebellum development head development regulation of nucleic acid-templated transcription regulation of transcription from RNA polymerase II promoter primitive erythrocyte differentiation
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