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LDHA Antibody (PA5-118027) in WB
Western Blot using LDHA Polyclonal Antibody (Product # PA5-118027) at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate, Lane B: NIH3T3 Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 37 kDa. (We are unsure of the identity of these extra bands).
Product Details
PA5-118027
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Mouse LDH-A/LDHA Protein
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2902631
Target Information
Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: L-lactate dehydrogenase; L-lactate dehydrogenase A chain; Lactate Dehydrogenase; lactate dehydrogenase 1, A chain; lactate dehydrogenase A4; lactate dehydrogenase-A; Lactic Dehydrogenase; LDH; LDH muscle subunit; LDH-A; LDH-M; M-LDH; OTTHUMP00000165206
Gene Aliases: l7R2; Ldh-1; Ldh1; Ldha; Ldhm
UniProt ID: (Mouse) P06151
Entrez Gene ID: (Mouse) 16828
Performance Guarantee
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