Proteintech

LDHA Monoclonal Antibody (2E2G6)

LDHA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

LDHA Antibody (66287-1-IG) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 66287-1-IG (LDHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

LDHA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

66287-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2E2G6

Immunogen

LDHA Fusion Protein Ag21417 (283-332 aa encoded by BC067223)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: IKDDVFLSV PCILGQNGIS DLVKVTLTSE EEARLKKSAD TLWGIQKELQ F (283-332 aa encoded by BC067223)

Target Information

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

OCT1 regulates the migration of colorectal cancer cells by acting on LDHA.

Histology and histopathology

Li L,Chen W,Wu G,Sun P

Mon Jan 01 00:00:00 UTC 2024

OCT1 regulates the migration of colorectal cancer cells by acting on LDHA.

Histology and histopathology

Li L,Chen W,Wu G,Sun P

Mon Jan 01 00:00:00 UTC 2024

Aerobic exercise reduced the amount of CHRONO bound to BMAL1 and ameliorated glucose metabolic dysfunction in skeletal muscle of high-fat diet-fed mice.

Life sciences

Xu L,Jia J,Miao S,Gong L,Wang J,He S,Zhang Y

Sat Jul 01 00:00:00 UTC 2023

Single-cell profiling reveals mechanisms of uncontrolled inflammation and glycolysis in decidual stromal cell subtypes in recurrent miscarriage.

Human reproduction (Oxford, England)

Bao S,Chen Z,Qin D,Xu H,Deng X,Zhang R,Ma J,Lu Z,Jiang S,Zhang X

Thu Jan 05 00:00:00 UTC 2023

FOXM1 regulates glycolysis in nasopharyngeal carcinoma cells through PDK1.

Journal of cellular and molecular medicine

Yang Q,Wu F,Zhang Y,Wang R

Fri Jul 01 00:00:00 UTC 2022

Lactate Dehydrogenase B Is Required for Pancreatic Cancer Cell Immortalization Through Activation of Telomerase Activity.

Frontiers in oncology

Wang R,Li J,Zhang C,Guan X,Qin B,Jin R,Qin L,Xu S,Zhang X,Liu R,Ye Q,Cheng L

Sat Jul 16 00:00:00 UTC 2022

Skeletal-Muscle-Specific Overexpression of Chrono Leads to Disruption of Glucose Metabolism and Exercise Capacity.

Life (Basel, Switzerland)

He S,Yan L,Zhu R,Wei H,Wang J,Zheng L,Zhang Y

Mon Aug 15 00:00:00 UTC 2022

LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis.

International journal of biological sciences

Fang Y,Dou R,Huang S,Han L,Fu H,Yang C,Song J,Zheng J,Zhang X,Liu K,Xiang Z,Zhang X,Wang S,Xiong B

Thu May 12 00:00:00 UTC 2022

Epigenomic and transcriptional profiling identifies impaired glyoxylate detoxification in NAFLD as a risk factor for hyperoxaluria.

Cell reports

Gianmoena K,Gasparoni N,Jashari A,Gabrys P,Grgas K,Ghallab A,Nordström K,Gasparoni G,Reinders J,Edlund K,Godoy P,Schriewer A,Hayen H,Hudert CA,Damm G,Seehofer D,Weiss TS,Boor P,Anders HJ,Motrapu M,Jansen P,Schiergens TS,Falk-Paulsen M,Rosenstiel P,Lisowski C,Salido E,Marchan R,Walter J,Hengstler JG,Cadenas C

Tue Aug 24 00:00:00 UTC 2021

Accumulated ROS Activates HIF-1α-Induced Glycolysis and Exerts a Protective Effect on Sensory Hair Cells Against Noise-Induced Damage.

Frontiers in molecular biosciences

Liang S,Dong S,Liu W,Wang M,Tian S,Ai Y,Wang H

Tue Feb 01 00:00:00 UTC 2022

Ursolic Acid Inhibits Breast Cancer Metastasis by Suppressing Glycolytic Metabolism via Activating SP1/Caveolin-1 Signaling.

Frontiers in oncology

Wang S,Chang X,Zhang J,Li J,Wang N,Yang B,Pan B,Zheng Y,Wang X,Ou H,Wang Z

Wed Sep 29 00:00:00 UTC 2021

Ursolic Acid Inhibits Breast Cancer Metastasis by Suppressing Glycolytic Metabolism via Activating SP1/Caveolin-1 Signaling.

Frontiers in oncology

Wang S,Chang X,Zhang J,Li J,Wang N,Yang B,Pan B,Zheng Y,Wang X,Ou H,Wang Z

Wed Sep 29 00:00:00 UTC 2021

Ursolic Acid Inhibits Breast Cancer Metastasis by Suppressing Glycolytic Metabolism via Activating SP1/Caveolin-1 Signaling.

Frontiers in oncology

Wang S,Chang X,Zhang J,Li J,Wang N,Yang B,Pan B,Zheng Y,Wang X,Ou H,Wang Z

Wed Sep 29 00:00:00 UTC 2021

Ursolic Acid Inhibits Breast Cancer Metastasis by Suppressing Glycolytic Metabolism via Activating SP1/Caveolin-1 Signaling.

Frontiers in oncology

Wang S,Chang X,Zhang J,Li J,Wang N,Yang B,Pan B,Zheng Y,Wang X,Ou H,Wang Z

Wed Sep 29 00:00:00 UTC 2021

Metabolomics reveals metabolite changes of patients with pulmonary arterial hypertension in China.

Journal of cellular and molecular medicine

Chen C,Luo F,Wu P,Huang Y,Das A,Chen S,Chen J,Hu X,Li F,Fang Z,Zhou S

Sat Feb 01 00:00:00 UTC 2020

LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma.

Bioscience reports

Hou XM,Yuan SQ,Zhao D,Liu XJ,Wu XA

Thu Jan 31 00:00:00 UTC 2019

LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma.

Bioscience reports

Hou XM,Yuan SQ,Zhao D,Liu XJ,Wu XA

Thu Jan 31 00:00:00 UTC 2019

MiR-323a-3p suppressed the glycolysis of osteosarcoma via targeting LDHA.

Human cell

Chen H,Gao S,Cheng C

Mon Oct 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; L-lactate dehydrogenase; L-lactate dehydrogenase A chain; Lactate Dehydrogenase; lactate dehydrogenase 1, A chain; lactate dehydrogenase A4; lactate dehydrogenase M; lactate dehydrogenase-A; Lactic Dehydrogenase; LDH; LDH muscle subunit; LDH-A; LDH-M; M-LDH; OTTHUMP00000165206; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59

View more View less

Gene Aliases: GSD11; HEL-S-133P; l7R2; Ldh-1; LDH-A; LDH-M; Ldh1; LDHA; LDHM; PIG19

View more View less

UniProt ID: (Human) P00338, (Mouse) P06151

View more View less

Entrez Gene ID: (Human) 3939, (Pig) 407245, (Mouse) 16828

View more View less

Function(s)

L-lactate dehydrogenase activity protein binding kinase binding identical protein binding NAD binding cadherin binding involved in cell-cell adhesion catalytic activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Process(es)

response to hypoxia lactate metabolic process pyruvate metabolic process glycolytic process response to nutrient response to glucose NAD metabolic process response to drug response to hydrogen peroxide positive regulation of apoptotic process response to estrogen post-embryonic organ development response to cAMP oxidation-reduction process cell-cell adhesion carbohydrate metabolic process lactate biosynthetic process from pyruvate carboxylic acid metabolic process cellular response to extracellular stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

LDHA Monoclonal Antibody (2E2G6)

LDHA Antibody (66287-1-IG) in ICC/IF

Product Details

66287-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!