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Invitrogen
LEFTY Polyclonal Antibody
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LEFTY Antibody (PA5-90431) in WB
Western blot analysis of LEFTY in extracts of various cell lines. Samples were incubated with LEFTY Polyclonal antibody (Product # PA5-90431) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-90431
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 77-366 of human LEFTY1 (NP_0662771)
Conjugate
Form
Liquid
Concentration
1.27 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806071
Product Specific Information
Immunogen sequence: RFSQSFREVA GRFLALEAST HLLVFGMEQR LPPNSELVQA VLRLFQEPVP KAALHRHGRL SPRSARARVT VEWLRVRDDG SNRTSLIDSR LVSVHESGWK AFDVTEAVNF WQQLSRPRQP LLLQVSVQRE HLGPLASGAH KLVRFASQGA PAGLGEPQLE LHTLDLGDYG AQGDCDPEAP MTEGTRCCRQ EMYIDLQGMK WAENWVLEPP GFLAYECVGT CRQPPEALAF KWPFLGPRQC IASETDSLPM IVSIKEGGRT RPQVVSLPNM RVQKCSCASD GALVPRRLQP; Positive Samples: HT-29, SW480, BxPC-3, Mouse pancreas, Mouse spleen, Rat pancreas
Target Information
This gene encodes a member of the TGF-beta family of proteins. A similar secreted protein in mouse plays a role in left-right asymmetry determination of organ systems during development. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Left-right determination factor 1; Left-right determination factor B; left-right determination, factor B; Lefty protein; Protein lefty-1; Protein lefty-B; Stimulated by retinoic acid gene 3 protein; TGF-beta-4; Transforming growth factor beta-4
Gene Aliases: AI450052; Ebaf; LEFTB; Lefty; lefty-1; LEFTY1; LEFTYB; Stra3; Tgfb4; UNQ278/PRO317
UniProt ID: (Human) O75610, (Mouse) Q64280
Entrez Gene ID: (Human) 10637, (Mouse) 13590
negative regulation of transcription from RNA polymerase II promoter
heart morphogenesis
multicellular organism development
determination of left/right symmetry
negative regulation of cell proliferation
anterior/posterior axis specification
positive regulation of pathway-restricted SMAD protein phosphorylation
cell growth
response to retinoic acid
growth
cell migration involved in gastrulation
regulation of apoptotic process
regulation of MAPK cascade
cell development
SMAD protein signal transduction
negative regulation of nodal signaling pathway
negative regulation of nodal receptor complex assembly
transforming growth factor beta receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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