Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
LEPREL1 Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
LEPREL1 Antibody (PA5-96918) in WB
Western blot analysis of LEPREL1 in extracts of NCI-H460 cells. Samples were incubated with LEPREL1 Polyclonal antibody (Product # PA5-96918) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96918
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human LEPREL1 (NP_0606622)
Conjugate
Form
Liquid
Concentration
0.47 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808720
Product Specific Information
Immunogen sequence: IRYGGRQDEN RVPSGVNVEG AEVHGFSMGK KLSPKIDRDL REGGPLLYEN ITFVYNSEQL NGTQRVLLDN VLSEEQCREL HSVASGIMLV GDGYRGKTSP HTPNEKFEGA TVLKALKSGY EGRVPLKSAR LFYDISEKAR RIVESYFMLN STLYFSYTHM VCRTALSGQQ DRRNDLSHPI HADNCLLDPE ANECWKEPPA YTFRDYSALL YMNDDFEGGE FIFTEMDAKT VTASIKPKCG RMISFSSGGE NPHGVKAVTK GKRCAVALWF TLDPLYRELE RIQADEVIAI LDQEQQGKHE L; Positive Samples: NCI-H460; Cellular Location: Endoplasmic reticulum, Golgi apparatus
Target Information
This gene encodes a member of the prolyl 3-hydroxylase subfamily of 2-oxo-glutarate-dependent dioxygenases. These enzymes play a critical role in collagen chain assembly, stability and cross-linking by catalyzing post-translational 3-hydroxylation of proline residues. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: leprecan-like 1; Leprecan-like protein 1; myxoid liposarcoma associated protein 4; Myxoid liposarcoma-associated protein 4; procollagen-proline 3-dioxygenase 2; Prolyl 3-hydroxylase 2; prolyl 3-hydroxylase 3
Gene Aliases: 4832416N06; AW553532; LEPREL1; MCVD; MLAT4; P3H2
UniProt ID: (Human) Q8IVL5, (Mouse) Q8CG71, (Rat) Q4KLM6
Entrez Gene ID: (Human) 55214, (Mouse) 210530, (Rat) 288016
iron ion binding
procollagen-proline 3-dioxygenase activity
L-ascorbic acid binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
metal ion binding
dioxygenase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support