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Invitrogen
LFG Polyclonal Antibody
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LFG Antibody (PA5-96520) in WB
Western blot analysis of LFG in mouse brain. Samples were incubated with LFG Polyclonal antibody (Product # PA5-96520) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96520
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human FAIM2 (NP_0364382)
Conjugate
Form
Liquid
Concentration
0.93 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808322
Product Specific Information
Immunogen sequence: MTQGKLSVAN KAPGTEGQQQ VHGEKKEAPA VPSAPPSYEE ATSGEGMKAG AFPPAPTAVP LHPSWAYVDP SSSSSYDNGF PTGDHELFTT FSWDDQKVRR VFVRK; Positive Samples: Mouse brain; Cellular Location: Cell junction, Cell membrane, Membrane raft, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Target Information
LFG, a seven transmembrane spanning protein, is a recently identified member of a family whose members can inhibit apoptotic cell death induced by Fas receptor. LFG is a human homolog of rat NMP35 and consists of a conserved transmembrane domain and a small cytoplasmic domain. Reports have shown that LFG protects the cell from Fas-mediated neuronal cell death in a novel way. LFG binds to Fas receptor but doesn't have any effect in reducing Fas expression or decreasing the ability of FADD binding to the receptor. Furthermore, LFG doesn't protect the cells from TNF-alpha mediated cell death process. Although LFG is ubiquitously expressed, Northern blot analysis detected an increased expression in hippocampus and other neural tissues, suggesting a protective role in neurodegenerative diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fas apoptotic inhibitory molecule 2; Neural membrane protein 35; protein lifeguard; Protein lifeguard 2
Gene Aliases: 2900002L20Rik; Faim2; Kiaa0950; Lfg; Lfg2; NMP25; Nmp35; Tmbim2
UniProt ID: (Mouse) Q8K097
Entrez Gene ID: (Mouse) 72393
response to ischemia
apoptotic process
cerebellum development
cerebellar Purkinje cell layer development
cerebellar granular layer development
cerebellar Purkinje cell differentiation
regulation of neuron apoptotic process
negative regulation of neuron apoptotic process
negative regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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