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LGMN Antibody (PA5-47271) in WB
Western blot analysis of LGMN in human kidney tissue, human heart tissue, and human placenta tissue. Samples were incubated in LGMN polyclonal antibody (Product # PA5-47271) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Specific bands were detected for Pro-Legumain/Asparaginyl Endopeptidase at approximately 56 kDa and mature Legumain/Asparaginyl Endopeptidase 37 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47271
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Legumain/Asparaginyl Endopeptidase Ile18-Tyr433
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605861
Product Specific Information
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AEP; Asparaginyl endopeptidase; cysteine protease 1; Legumain; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1EC 3.4.22.34
Gene Aliases: AEP; LGMN; LGMN1; PRSC1
UniProt ID: (Human) Q99538
Entrez Gene ID: (Human) 5641
toll-like receptor signaling pathway
renal system process
proteolysis
vacuolar protein processing
response to acidic pH
antigen processing and presentation of exogenous peptide antigen via MHC class II
receptor catabolic process
negative regulation of multicellular organism growth
vitamin D metabolic process
negative regulation of neuron apoptotic process
proteolysis involved in cellular protein catabolic process
negative regulation of ERBB signaling pathway
Performance Guarantee
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