Proteintech

LGTN Polyclonal Antibody

LGTN Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

LGTN Antibody (12840-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using LGTN antibody 12840-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Product Details

12840-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LGTN Fusion Protein Ag3579 (235-585 aa encoded by BC001585)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DKSLSEAPE DTSTRGLNQD STDSKTLQEQ MDELLQQCFL HALKCRVKKA DLPLLTSTFL GSHMFSCCPE GRQLDIKKSS YKKLSKFLQQ MQQEQIIQVK ELSKGVESIV AVDWKHPRIT SFVIPEPSPT SQTIQEGSRE QPYHPPDIKP LYCVPASMTL LFQESGHKKG SFLEGSEVRT IVINYAKKND LVDADNKNLV RLDPILCDCI LEKNEQHTVM KLPWDSLLTR CLEKLQPAYQ VTLPGQEPIV KKGRICPIDI TLAQRASNKK VTVVRNLEAY GLDPYSVAAI LQQRCQASTT VNPAPGAKDS LQVQIQGNQV HHLGWLLLEE YQLPRKHIQG LEKALKPGKK K (235-585 aa encoded by BC001585)

Target Information

This gene encodes a translation initiation factor involved in the recruitment and delivery of aminoacyl-tRNAs to the P-site of the eukaryotic ribosome in a GTP-independent manner. This gene was previously referred to as ligatin, but is now known to localize to the cytoplasm and localize and function with translation factors. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Relocalization of Translation Termination and Ribosome Recycling Factors to Stress Granules Coincides with Elevated Stop-Codon Readthrough and Reinitiation Rates upon Oxidative Stress.

Cells

Makeeva DS,Riggs CL,Burakov AV,Ivanov PA,Kushchenko AS,Bykov DA,Popenko VI,Prassolov VS,Ivanov PV,Dmitriev SE

Sun Jan 08 00:00:00 UTC 2023

The enterovirus genome can be translated in an IRES-independent manner that requires the initiation factors eIF2A/eIF2D.

PLoS biology

Kim H,Aponte-Diaz D,Sotoudegan MS,Shengjuler D,Arnold JJ,Cameron CE

Sun Jan 01 00:00:00 UTC 2023

Identification and characterization of novel proteins associated with CHD4.

Genes to cells : devoted to molecular & cellular mechanisms

Sakaguchi C,Ichihara K,Nita A,Katayama Y,Nakayama KI

Sat Jan 01 00:00:00 UTC 2022

Non-canonical initiation factors modulate repeat-associated non-AUG translation.

Human molecular genetics

Green KM,Miller SL,Malik I,Todd PK

Wed Aug 17 00:00:00 UTC 2022

Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons.

Nucleic acids research

Ichihara K,Matsumoto A,Nishida H,Kito Y,Shimizu H,Shichino Y,Iwasaki S,Imami K,Ishihama Y,Nakayama KI

Wed Jul 21 00:00:00 UTC 2021

Local translation in nuclear condensate amyloid bodies.

Proceedings of the National Academy of Sciences of the United States of America

Theodoridis PR,Bokros M,Marijan D,Balukoff NC,Wang D,Kirk CC,Budine TD,Goldsmith HD,Wang M,Audas TE,Lee S

Tue Feb 16 00:00:00 UTC 2021

Translation inhibitory elements from Hoxa3 and Hoxa11 mRNAs use uORFs for translation inhibition.

eLife

Alghoul F,Laure S,Eriani G,Martin F

Wed Jun 02 00:00:00 UTC 2021

A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation.

Nature communications

Sonobe Y,Aburas J,Krishnan G,Fleming AC,Ghadge G,Islam P,Warren EC,Gu Y,Kankel MW,Brown AEX,Kiskinis E,Gendron TF,Gao FB,Roos RP,Kratsios P

Fri Oct 15 00:00:00 UTC 2021

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Molecular cell

Marmor-Kollet H,Siany A,Kedersha N,Knafo N,Rivkin N,Danino YM,Moens TG,Olender T,Sheban D,Cohen N,Dadosh T,Addadi Y,Ravid R,Eitan C,Toth Cohen B,Hofmann S,Riggs CL,Advani VM,Higginbottom A,Cooper-Knock J,Hanna JH,Merbl Y,Van Den Bosch L,Anderson P,Ivanov P,Geiger T,Hornstein E

Thu Dec 03 00:00:00 UTC 2020

The Initiation Factors eIF2, eIF2A, eIF2D, eIF4A, and eIF4G Are Not Involved in Translation Driven by Hepatitis C Virus IRES in Human Cells.

Frontiers in microbiology

González-Almela E,Williams H,Sanz MA,Carrasco L

Wed Sep 30 00:00:00 UTC 2020

The Initiation Factors eIF2, eIF2A, eIF2D, eIF4A, and eIF4G Are Not Involved in Translation Driven by Hepatitis C Virus IRES in Human Cells.

Frontiers in microbiology

González-Almela E,Williams H,Sanz MA,Carrasco L

Wed Sep 30 00:00:00 UTC 2020

Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B.

Cell reports

Ho JJD,Balukoff NC,Cervantes G,Malcolm PD,Krieger JR,Lee S

Tue Jan 02 00:00:00 UTC 2018

Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B.

Cell reports

Ho JJD,Balukoff NC,Cervantes G,Malcolm PD,Krieger JR,Lee S

Tue Jan 02 00:00:00 UTC 2018

Translation of Sindbis Subgenomic mRNA is Independent of eIF2, eIF2A and eIF2D.

Scientific reports

Sanz MA,González Almela E,Carrasco L

Mon Feb 27 00:00:00 UTC 2017

Translation of Sindbis Subgenomic mRNA is Independent of eIF2, eIF2A and eIF2D.

Scientific reports

Sanz MA,González Almela E,Carrasco L

Mon Feb 27 00:00:00 UTC 2017

Translation of Sindbis Subgenomic mRNA is Independent of eIF2, eIF2A and eIF2D.

Scientific reports

Sanz MA,González Almela E,Carrasco L

Mon Feb 27 00:00:00 UTC 2017

Translation of Sindbis Subgenomic mRNA is Independent of eIF2, eIF2A and eIF2D.

Scientific reports

Sanz MA,González Almela E,Carrasco L

Mon Feb 27 00:00:00 UTC 2017

Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.

Science (New York, N.Y.)

Starck SR,Jiang V,Pavon-Eternod M,Prasad S,McCarthy B,Pan T,Shastri N

Fri Jun 29 00:00:00 UTC 2012

Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.

Science (New York, N.Y.)

Starck SR,Jiang V,Pavon-Eternod M,Prasad S,McCarthy B,Pan T,Shastri N

Fri Jun 29 00:00:00 UTC 2012

Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling.

Genes & development

Skabkin MA,Skabkina OV,Dhote V,Komar AA,Hellen CU,Pestova TV

Sun Aug 15 00:00:00 UTC 2010

Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling.

Genes & development

Skabkin MA,Skabkina OV,Dhote V,Komar AA,Hellen CU,Pestova TV

Sun Aug 15 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: eIF2D; Eukaryotic translation initiation factor 2D; Hepatocellular carcinoma-associated antigen 56; Ligatin

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Gene Aliases: D1Ertd5e; EIF2D; HCA56; LGTN

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UniProt ID: (Human) P41214, (Rat) Q5PPG7, (Mouse) Q61211

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Entrez Gene ID: (Human) 1939, (Rat) 498225, (Mouse) 16865

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Function(s)

translation initiation factor activity receptor activity RNA binding

Process(es)

formation of translation preinitiation complex IRES-dependent translational initiation intracellular protein transport ribosome disassembly translation translational initiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LGTN Polyclonal Antibody

LGTN Antibody (12840-1-AP) in ICC/IF

Product Details

12840-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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