Invitrogen

LHX2 Polyclonal Antibody

1 Published Figure

2 References

View all (32) LHX2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (1)

LHX2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

FIGURE: 1 / 6

LHX2 Antibody (PA5-78287) in IHC (F)

LHX2 Polyclonal Antibody detects LHX2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: LHX2 protein stained by LHX2 Polyclonal Antibody (Product # PA5-78287) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .

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Product Details

PA5-78287

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Fish, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LHX2. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.97 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2736088

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, mouse brain, mouse eye

Predicted Reactivity: Pig (98%), Chicken (81%), Rhesus Monkey (98%), Bovine (98%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DNA dioxygenases Tet2/3 regulate gene promoter accessibility and chromatin topology in lineage-specific loci to control epithelial differentiation.

Science advances

Chen GD,Fatima I,Xu Q,Rozhkova E,Fessing MY,Mardaryev AN,Sharov AA,Xu GL,Botchkarev VA

PA5-78287 was used in Immunohistochemistry to demonstrate the essential roles for Tet2/3 in establishment of lineage-specific gene expression program and control of Dlx3/Krt25/Krt28 axis in hair follicle epithelial cells and implicate modulation of DNA methylation as a novel approach for hair growth control.

Fri Jan 13 00:00:00 UTC 2023

Chromosome 10q26-driven age-related macular degeneration is associated with reduced levels of HTRA1 in human retinal pigment epithelium.

Proceedings of the National Academy of Sciences of the United States of America

Williams BL,Seager NA,Gardiner JD,Pappas CM,Cronin MC,Amat di San Filippo C,Anstadt RA,Liu J,Toso MA,Nichols L,Parnell TJ,Eve JR,Heinz S,Hayes MGB,Bartel PL,Zouache MA,Richards BT,Hageman GS

PA5-78287 was used in Gel Shift, Immunohistochemistry (Frozen), Western Blot to show that HTRA1 messenger RNA (mRNA) is reduced in retinal pigment epithelium (RPE) but not in neural retina or choroid tissues derived from human donors with homozygous risk at the 10q26 locus.

Tue Jul 27 00:00:00 UTC 2021

Chromosome 10q26-driven age-related macular degeneration is associated with reduced levels of HTRA1 in human retinal pigment epithelium.

Proceedings of the National Academy of Sciences of the United States of America

Williams BL,Seager NA,Gardiner JD,Pappas CM,Cronin MC,Amat di San Filippo C,Anstadt RA,Liu J,Toso MA,Nichols L,Parnell TJ,Eve JR,Heinz S,Hayes MGB,Bartel PL,Zouache MA,Richards BT,Hageman GS

Tue Jul 27 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: Homeobox protein LH-2; LIM homeo box protein 2; LIM homeobox protein 2; LIM HOX gene 2; LIM/homeobox protein Lhx2; MGC138390

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Gene Aliases: ap; apterous; hLhx2; Lh-2; LH2; LH2A; LHX2; Lim2

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UniProt ID: (Human) P50458, (Mouse) Q9Z0S2

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Entrez Gene ID: (Human) 9355, (Mouse) 16870, (Rat) 296706

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Function(s)

transcription factor activity, RNA polymerase II transcription factor binding chromatin binding zinc ion binding sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding DNA binding protein binding metal ion binding

Process(es)

neural tube closure hair follicle development transcription, DNA-templated axon guidance mesoderm development dorsal/ventral pattern formation olfactory bulb development telencephalon regionalization cerebral cortex development maintenance of epithelial cell apical/basal polarity positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter axon extension retina development in camera-type eye negative regulation of transcription regulatory region DNA binding regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter nervous system development brain development telencephalon development neurogenesis neuron differentiation anatomical structure formation involved in morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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LHX2 Polyclonal Antibody

LHX2 Antibody (PA5-78287) in IHC (F)

Product Details

PA5-78287

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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