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Invitrogen
LIG4 Polyclonal Antibody
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FIGURE: 1 / 2
LIG4 Antibody (PA5-109911) in IHC (P)
Immunohistochemistry analysis of LIG4 in paraffin-embedded human colon carcinoma. Samples were incubated with LIG4 Polyclonal antibody (Product # PA5-109911) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
PA5-109911
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DNA Ligase IV (NP_0023032)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2855322
Product Specific Information
Immunogen sequence: MAASQTSQTV ASHVPFADLC STLERIQKSK GRAEKIRHFR EFLDSWRKFH DALHKNHKDV TDSFYPAMRL ILPQLERERM AYGIKETMLA KLYIELLNLP RDGKDALKLL NYRTPTGTHG DAGDFAMIAY FVLKPRCLQK GSLTIQQVND LLDSIASNNS AKRKDLIKKS LLQLITQSSA LEQKWLIRMI IKDLKLGVSQ QTIFSVFHND
Target Information
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA joinase; DNA ligase (ATP) 4; DNA ligase 4; DNA ligase IV; DNA repair enzyme; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase
Gene Aliases: 5830471N16Rik; LIG4; LIG4S; tiny
UniProt ID: (Human) P49917, (Mouse) Q8BTF7
Entrez Gene ID: (Human) 3981, (Rat) 290907, (Mouse) 319583
single strand break repair
in utero embryonic development
pro-B cell differentiation
DNA ligation
lagging strand elongation
DNA repair
nucleotide-excision repair
nucleotide-excision repair, DNA gap filling
double-strand break repair
double-strand break repair via nonhomologous end joining
central nervous system development
cell proliferation
response to X-ray
response to ionizing radiation
response to gamma radiation
T cell differentiation in thymus
V(D)J recombination
immunoglobulin V(D)J recombination
T cell receptor V(D)J recombination
somatic stem cell population maintenance
negative regulation of neuron apoptotic process
isotype switching
positive regulation of fibroblast proliferation
positive regulation of neurogenesis
DNA ligation involved in DNA recombination
DNA ligation involved in DNA repair
chromosome organization
neuron apoptotic process
cellular response to lithium ion
DNA biosynthetic process
double-strand break repair via classical nonhomologous end joining
DNA replication
DNA recombination
cellular response to DNA damage stimulus
cell cycle
cell division
establishment of integrated proviral latency
positive regulation of chromosome organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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