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LIGHT Antibody (PA5-144654) in IHC (P)
Immunohistochemistry of LIGHT in paraffin-embedded human kidney tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with LIGHT polyclonal antibody (Product # PA5-144654) with a dilution of 1:600 for 1 hour at room temperature. ... View More
Product Details
PA5-144654
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human TNFSF14 aa 190-240/240.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3093617
Product Specific Information
Positive control: Mouse liver tissue lysates, human liver tissue, human colon tissue, human kidney tissue.
Predicted band size: 26 kDa
Subcellular Location: Cell membrane, Secreted, Cytoplasm.
Target Information
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD258; Herpes virus entry mediator ligand; herpesvirus entry mediator ligand; HVEM-L; sCD258; sLIGHT; soluble CD258; soluble LIGHT; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand 1D; Tumor necrosis factor ligand superfamily member 14
Gene Aliases: CD258; HVEM-L; HVEML; LIGHT; LTg; Ly113; TNFSF14; UNQ391/PRO726
UniProt ID: (Human) O43557, (Mouse) Q9QYH9
Entrez Gene ID: (Human) 8740, (Mouse) 50930
immune response
release of cytoplasmic sequestered NF-kappaB
positive regulation of T cell chemotaxis
T cell costimulation
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of myoblast differentiation
positive regulation of myoblast fusion
apoptotic process
signal transduction
tumor necrosis factor-mediated signaling pathway
T cell proliferation
T cell activation
T cell homeostasis
cellular response to mechanical stimulus
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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