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LIGHT Antibody (PA5-19190) in ICC/IF
Immunocytochemistry analysis of LIGHT using LIGHT Polyclonal Antibody (Product # PA5-19190) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-19190
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (HEVNPAAHLTGANS) corresponding to the internal amino acids of TNFSF14 (aa 90-104).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10979775
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
Target Information
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD258; Herpes virus entry mediator ligand; herpesvirus entry mediator ligand; HVEM-L; sCD258; sLIGHT; soluble CD258; soluble LIGHT; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand 1D; Tumor necrosis factor ligand superfamily member 14
Gene Aliases: CD258; HVEML; LIGHT; LTg; TNFSF14; UNQ391/PRO726
UniProt ID: (Human) O43557
Entrez Gene ID: (Human) 8740
apoptotic process
immune response
signal transduction
release of cytoplasmic sequestered NF-kappaB
positive regulation of T cell chemotaxis
T cell costimulation
tumor necrosis factor-mediated signaling pathway
T cell proliferation
T cell activation
T cell homeostasis
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of myoblast differentiation
cellular response to mechanical stimulus
positive regulation of myoblast fusion
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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