Invitrogen

LIM1 Polyclonal Antibody

View all (44) LIM1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-116485

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Flow Cytometry (Flow)

1:50-1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within zebrafish LIM1 aa 144-193
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2901116

Product Specific Information

Positive controls: A431 cell lysate, zebrafish tissue lysate, rat brian tissue, mouse brian tissue, MCF-7.

Target Information

This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of neural and lymphoid cells. A similar protein in mice is an essential regulator of the vertebrate head organizer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: hLim-1; Homeobox protein Lim-1; LHX-1; LIM homeo box protein 1; LIM homeobox protein 1; LIM/homeobox protein Lhx1; lim1a; MGC126723; MGC138141; Rlim

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Gene Aliases: LHX1; lhx1a; LIM-1; LIM1; wu:fe25d04; wu:fj36b06

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UniProt ID: (Human) P48742, (Mouse) P63006, (Rat) P63007, (Zebrafish) Q90476

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Entrez Gene ID: (Human) 3975, (Mouse) 16869, (Rat) 257634, (Zebrafish) 30463

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Function(s)
transcription factor activity, sequence-specific DNA binding transcription corepressor activity zinc ion binding sequence-specific DNA binding DNA binding protein binding metal ion binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding
Process(es)
ureteric bud development ectoderm formation endoderm formation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development motor neuron axon guidance post-embryonic development embryonic pattern specification dorsal/ventral pattern formation retina layer formation spinal cord association neuron differentiation cerebellar Purkinje cell differentiation forebrain regionalization cerebellar Purkinje cell-granule cell precursor cell signaling involved in regulation of granule cell precursor cell proliferation neuron differentiation positive regulation of embryonic development negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic retina morphogenesis in camera-type eye head development paramesonephric duct development comma-shaped body morphogenesis S-shaped body morphogenesis renal vesicle morphogenesis nephric duct morphogenesis primitive streak formation positive regulation of branching involved in ureteric bud morphogenesis dorsal spinal cord interneuron posterior axon guidance lateral motor column neuron migration positive regulation of gastrulation positive regulation of anterior head development positive regulation of nephron tubule epithelial cell differentiation urogenital system development branching involved in ureteric bud morphogenesis gastrulation with mouth forming second kidney development cell-cell signaling pattern specification process nervous system development anatomical structure morphogenesis organ morphogenesis anterior/posterior axis specification anterior/posterior pattern specification regulation of gene expression ventral spinal cord development telencephalon development cerebellum development somite rostral/caudal axis specification metanephric part of ureteric bud development oviduct epithelium development uterine epithelium development nephric duct elongation horizontal cell localization cellular response to fibroblast growth factor stimulus anatomical structure formation involved in morphogenesis embryonic viscerocranium morphogenesis pronephros development uterus development oviduct development cervix development vagina development epithelium development mesonephric duct development metanephric glomerulus development metanephric comma-shaped body morphogenesis metanephric renal vesicle morphogenesis metanephric S-shaped body morphogenesis transcription, DNA-templated cell differentiation renal system development mesonephric tubule development
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