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LIM2 Antibody (PA5-121153) in WB
Western blot analysis of LIM2 in extracts of various cell lines. Samples were incubated with LIM2 Polyclonal antibody (Product # PA5-121153) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-121153
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-110 of human LIM2 (NP_085915.2).
Conjugate
Form
Liquid
Concentration
2.17 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914725
Product Specific Information
Positive test controls include: BT-474, HepG2, Jurkat, Mouse liver, Mouse eye, Rat liver. The target is usually found in the following locations: Membrane, Multi-pass membrane protein.
Immunogen sequence: TDHWMQYRLS GSFAHQGLWR YCLGNKCYLQ TDSIGEPPGQ GPGRAWGKSR ADLGAQGHLY SRWRTLRLKE GKGATQAYWN ATRAFM
Target Information
This gene encodes an eye lens-specific protein found at the junctions of lens fiber cells, where it may contribute to cell junctional organization. It acts as a receptor for calmodulin, and may play an important role in both lens development and cataractogenesis. Mutations in this gene have been associated with cataract formation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Lens fiber membrane intrinsic protein; lens intrinsic membrane protein 2, 19kDa; MP17; MP18; MP19; MP20; total opacity 3
Gene Aliases: 19kDa; 4833403J20; CTRCT19; LIM2; MP17; MP19; MP20; To3
UniProt ID: (Human) P55344, (Mouse) P56563, (Rat) P54825
Entrez Gene ID: (Human) 3982, (Mouse) 233187, (Rat) 114903
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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