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Recommended positive controls: H1299, HeLaS3, mouse brain.
Predicted reactivity: Mouse (80%), Bovine (86%).
IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: LIM and calponin homology domains-containing protein 1
Gene Aliases: 3732412D22Rik; KIAA1102; LIMCH1; LIMCH1A; LMO7B; mKIAA1102
UniProt ID: (Human) Q9UPQ0, (Mouse) Q3UH68
Entrez Gene ID: (Human) 22998, (Mouse) 77569
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